| 产品: | UBA3 抗体 |
| 货号: | DF8358 |
| 描述: | Rabbit polyclonal antibody to UBA3 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 49 kDa; 52kD(Calculated). |
| 蛋白号: | Q8TBC4 |
| RRID: | AB_2841623 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8358, RRID:AB_2841623.
展开/折叠
DKFZp566J164; EC 6.3.2.; hUba3; MGC22384; NEDD8 activating enzyme E1 catalytic subunit; NEDD8 activating enzyme E1C; Nedd8 activating enzyme hUba3; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; uba3; UBA3 ubiquitin activating enzyme E1 homolog; UBA3_HUMAN; UBE1C; Ubiquitin activating enzyme 3; Ubiquitin activating enzyme E1C; Ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3;
抗原和靶标
- Q8TBC4 UBA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TBC4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K9 | Methylation | Uniprot | |
| K10 | Methylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| R135 | Methylation | Uniprot | |
| K147 | Sumoylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K284 | Sumoylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| R295 | Methylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| K409 | Acetylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot |
研究背景
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Ubiquitously expressed.
Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand (By similarity). Interacts with TBATA (By similarity).
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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