产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8373, RRID:AB_2841635.
展开/折叠
Axam2; DKFZp762A2316; KIAA1331; Senp2; SENP2_HUMAN; Sentrin specific protease 2; Sentrin-specific protease 2; Sentrin/SUMO specific protease SENP2; Sentrin/SUMO-specific protease SENP2; SMT3 specific isopeptidase 2; SMT3-specific isopeptidase 2; Smt3ip2; SUMO1/sentrin/SMT3 specific peptidase 2;
抗原和靶标
- Q9HC62 SENP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYRWLVRILGTIFRFCDRSVPPARALLKRRRSDSTLFSTVDTDEIPAKRPRLDCFIHQVKNSLYNAASLFGFPFQLTTKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNMLKLGNKSPNGISDYPKIRVTVTRDQPRRVLPSFGFTLNSEGCNRRPGGRRHSKGNPESSLMWKPQEQAVTEMISEESGKGLRRPHCTVEEGVQKEEREKYRKLLERLKESGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVPKQYRLVETRGPLCSLRSEKRCSKGKITDTETMVGIRFENESRRGYQLEPDLSEEVSARLRLGSGSNGLLRRKVSIIETKEKNCSGKERDRRTDDLLELTEDMEKEISNALGHGPQDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYMNLLVERNKKQGYPALHVFSTFFYPKLKSGGYQAVKRWTKGVNLFEQEIILVPIHRKVHWSLVVIDLRKKCLKYLDSMGQKGHRICEILLQYLQDESKTKRNSDLNLLEWTHHSMKPHEIPQQLNGSDCGMFTCKYADYISRDKPITFTQHQMPLFRKKMVWEILHQQLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9HC62 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| Y130 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K271 | Acetylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| T369 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| K455 | Ubiquitination | Uniprot | |
| Y493 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| Y558 | Phosphorylation | Uniprot |
研究背景
Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1. Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB.
Polyubiquitinated; which leads to proteasomal degradation.
Nucleus>Nuclear pore complex. Nucleus membrane>Peripheral membrane protein>Nucleoplasmic side. Cytoplasm.
Note: Shuttles between cytoplasm and nucleus.
Binds to SUMO2 and SUMO3 (By similarity). Interacts with the C-terminal domain of NUP153 via its N-terminus. Interacts with MTA1.
The N-terminus is necessary and sufficient for nuclear envelope targeting.
Belongs to the peptidase C48 family.
研究领域
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
限制条款
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