| 产品: | ENC1 抗体 |
| 货号: | DF8407 |
| 描述: | Rabbit polyclonal antibody to ENC1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 66 kDa; 66kD(Calculated). |
| 蛋白号: | O14682 |
| RRID: | AB_2841658 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8407, RRID:AB_2841658.
展开/折叠
CCL 28; CCL28; Ectoderm neural cortex protein 1; Ectoderm-neural cortex protein 1; Ectodermal neural cortex (with BTB like domain); ENC 1; ENC-1; ENC1; ENC1_HUMAN; FLJ39259; Kelch like 35; Kelch like 37; Kelch-like protein 37; KLHL 35; KLHL35; KLHL37; NRPB; Nuclear matrix protein NRP/B; Nuclear restricted protein BTB domain like (brain); OTTHUMP00000128235; OTTHUMP00000222054; p53 induced gene 10 protein; p53-induced gene 10 protein; PIG 10; PIG10; TP53I10; Tumor protein p53 inducible protein 10;
抗原和靶标
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. May be down-regulated in neuroblastoma tumors.
- O14682 ENC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVSVHENRKSRASSGSINIYLFHKSSYADSVLTHLNLLRQQRLFTDVLLHAGNRTFPCHRAVLAACSRYFEAMFSGGLKESQDSEVNFDNSIHPEVLELLLDYAYSSRVIINEENAESLLEAGDMLEFQDIRDACAEFLEKNLHPTNCLGMLLLSDAHQCTKLYELSWRMCLSNFQTIRKNEDFLQLPQDMVVQLLSSEELETEDERLVYESAINWISYDLKKRYCYLPELLQTVRLALLPAIYLMENVAMEELITKQRKSKEIVEEAIRCKLKILQNDGVVTSLCARPRKTGHALFLLGGQTFMCDKLYLVDQKAKEIIPKADIPSPRKEFSACAIGCKVYITGGRGSENGVSKDVWVYDTLHEEWSKAAPMLVARFGHGSAELKHCLYVVGGHTAATGCLPASPSVSLKQVEHYDPTINKWTMVAPLREGVSNAAVVSAKLKLFAFGGTSVSHDKLPKVQCYDQCENRWTVPATCPQPWRYTAAAVLGNQIFIMGGDTEFSACSAYKFNSETYQWTKVGDVTAKRMSCHAVASGNKLYVVGGYFGIQRCKTLDCYDPTLDVWNSITTVPYSLIPTAFVSTWKHLPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14682 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| T452 | Phosphorylation | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| T569 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| Y573 | Phosphorylation | Uniprot |
研究背景
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism.
Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination.
Nucleus matrix. Cytoplasm. Cytoplasm>Cytoskeleton.
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. May be down-regulated in neuroblastoma tumors.
Binds to RB1. Hypophosphorylated RB1 associates with ENC1 during neuronal differentiation, while hyperphosphorylated RB1 associates with ENC1 in undifferentiating cells. Part of a complex that contains CUL3, RBX1 and ENC1. Interacts indirectly with KEAP1.
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