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NUP98 Antibody - Western blot analysis NUP98 using MCF7 whole cell lysates.

NUP98 Antibody - DF8408 staining A549 cells by IF/ICC.

产品:	NUP98 抗体
货号:	DF8408
描述:	Rabbit polyclonal antibody to NUP98
应用:	WB IF/ICC
反应:	Human
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	188 kDa; 198kD(Calculated).
蛋白号:	P52948
RRID:	AB_2841659

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

预测:

Pig(92%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)

克隆:

Polyclonal

特异性:

NUP98 Antibody detects endogenous levels of total NUP98.

RRID:

AB_2841659
引用格式: Affinity Biosciences Cat# DF8408, RRID:AB_2841659.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

96 kDa nucleoporin; 98 kDa nucleoporin; ADAR2; ADIR2; GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup96; Nuclear pore complex protein Nup98 Nup96; Nucleoporin 98kD; nucleoporin 98kDa; Nucleoporin Nup96; Nucleoporin Nup98; NUP196; NUP96; Nup98; Nup98-Nup96; NUP98_HUMAN;

抗原和靶标

免疫原:

Uniprot:

P52948(NUP98_HUMAN) >>Visit HPA database.

基因/基因ID:

NUP98(4928)

序列:

P52948 NUP98_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAGTTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQTTSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGNKPTLTLGTNTNTSNFGFGTNTSGNSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTATLGFGAPQAPVALTDPNASAAQQAVLQQHINSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSENLASPSEYPENGERFSFLSKPVDENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMRAALRNGLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVVVYLDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVLDTMLEESMPEDQEPVSASTHIASSLGINPHVLQIMKASLLTDEEDVDMALDQRFSRLPSKADTSQEICSPRLPISASHSSKTRSLVGGLLQSKFTSGAFLSPSVSVQECRTPRAASLMNIPSTSSWSVPPPLTSVFTMPSPAPEVPLKTVGTRRQLGLVPREKSVTYGKGKLLMDMALFMGRSFRVGWGPNWTLANSGEQLNGSHELENHQIADSMEFGFLPNPVAVKPLTESPFKVHLEKLSLRQRKPDEDMKLYQTPLELKLKHSTVHVDELCPLIVPNLGVAVIHDYADWVKEASGDLPEAQIVKHWSLTWTLCEALWGHLKELDSQLNEPREYIQILERRRAFSRWLSCTATPQIEEEVSLTQKNSPVEAVFSYLTGKRISEACSLAQQSGDHRLALLLSQFVGSQSVRELLTMQLVDWHQLQADSFIQDERLRIFALLAGKPVWQLSEKKQINVCSQLDWKRSLAIHLWYLLPPTASISRALSMYEEAFQNTSDSDRYACSPLPSYLEGSGCVIAEEQNSQTPLRDVCFHLLKLYSDRHYDLNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLSAQCEGVLQASYAGQLESEGLWEWAIFVLLHIDNSGIREKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHMESDKHLEALCLFKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYIRVIEMLRHIQQVDCSGNDLEQLHIKVTSLCSRIEQIQCYSAKDRLAQSDMAKRVANLLRVVLSLHHPPDRTSDSTPDPQRVPLRLLAPHIGRLPMPEDYAMDELRSLTQSYLRELAVGSL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Pig

Chicken

Rabbit

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52948 作为底物

P52948

Site	PTM Type	Enzyme	Source
T168	Phosphorylation		Uniprot
T170	Phosphorylation		Uniprot
T172	Phosphorylation		Uniprot
K175	Ubiquitination		Uniprot
S179	Phosphorylation		Uniprot
T184	O-Glycosylation		Uniprot
K185	Ubiquitination		Uniprot
T190	Phosphorylation		Uniprot
K193	Ubiquitination		Uniprot
K198	Ubiquitination		Uniprot
R204	Methylation		Uniprot
K213	Ubiquitination		Uniprot
S511	Phosphorylation		Uniprot
S516	Phosphorylation		Uniprot
S524	Phosphorylation		Uniprot
K534	Ubiquitination		Uniprot
T536	Phosphorylation		Uniprot
K542	Ubiquitination		Uniprot
T545	Phosphorylation		Uniprot
T546	Phosphorylation		Uniprot
T548	Phosphorylation		Uniprot
Y550	Phosphorylation		Uniprot
K551	Ubiquitination		Uniprot
T553	Phosphorylation		Uniprot
K563	Ubiquitination		Uniprot
T567	O-Glycosylation		Uniprot
T568	O-Glycosylation		Uniprot
T568	Phosphorylation		Uniprot
K572	Acetylation		Uniprot
K572	Methylation		Uniprot
K572	Ubiquitination		Uniprot
K594	Acetylation		Uniprot
K594	Sumoylation		Uniprot
S596	Phosphorylation		Uniprot
K603	Acetylation		Uniprot
K603	Ubiquitination		Uniprot
S608	Phosphorylation	Q9HC98 (NEK6)	Uniprot
S612	Phosphorylation	P24941 (CDK2) , P06493 (CDK1)	Uniprot
S618	Phosphorylation		Uniprot
S623	Phosphorylation	P06493 (CDK1)	Uniprot
S625	Phosphorylation		Uniprot
Y627	Phosphorylation		Uniprot
S635	Phosphorylation		Uniprot
S638	Phosphorylation		Uniprot
K639	Ubiquitination		Uniprot
S653	Phosphorylation		Uniprot
Y659	Phosphorylation		Uniprot
K665	Ubiquitination		Uniprot
T670	Phosphorylation	P06493 (CDK1)	Uniprot
S673	Phosphorylation		Uniprot
K677	Ubiquitination		Uniprot
S679	Phosphorylation		Uniprot
S681	Phosphorylation		Uniprot
S683	Phosphorylation		Uniprot
T687	Phosphorylation		Uniprot
S704	Phosphorylation		Uniprot
S714	Phosphorylation		Uniprot
R719	Methylation		Uniprot
S726	Phosphorylation		Uniprot
Y741	Phosphorylation		Uniprot
Y742	Phosphorylation		Uniprot
T743	Phosphorylation		Uniprot
S746	Phosphorylation		Uniprot
K752	Ubiquitination		Uniprot
K757	Ubiquitination		Uniprot
K808	Ubiquitination		Uniprot
K818	Sumoylation		Uniprot
K818	Ubiquitination		Uniprot
K831	Ubiquitination		Uniprot
K838	Ubiquitination		Uniprot
S839	Phosphorylation	Q9HC98 (NEK6)	Uniprot
R842	Methylation		Uniprot
Y848	Phosphorylation		Uniprot
S856	Phosphorylation		Uniprot
K858	Ubiquitination		Uniprot
K864	Ubiquitination		Uniprot
S888	Phosphorylation		Uniprot
S897	Phosphorylation		Uniprot
T899	Phosphorylation		Uniprot
S900	Phosphorylation		Uniprot
S913	Phosphorylation		Uniprot
T916	Phosphorylation		Uniprot
K925	Ubiquitination		Uniprot
S932	Phosphorylation		Uniprot
S934	Phosphorylation		Uniprot
S948	Phosphorylation		Uniprot
S966	Phosphorylation		Uniprot
S997	Phosphorylation		Uniprot
T1000	Phosphorylation		Uniprot
S1014	Phosphorylation		Uniprot
S1018	Phosphorylation		Uniprot
K1019	Sumoylation		Uniprot
K1019	Ubiquitination		Uniprot
T1022	Phosphorylation		Uniprot
S1023	Phosphorylation		Uniprot
S1028	Phosphorylation		Uniprot
S1034	Phosphorylation		Uniprot
S1036	Phosphorylation		Uniprot
S1038	Phosphorylation		Uniprot
K1040	Ubiquitination		Uniprot
T1041	Phosphorylation		Uniprot
S1043	Phosphorylation		Uniprot
K1052	Ubiquitination		Uniprot
T1054	Phosphorylation		Uniprot
S1055	Phosphorylation		Uniprot
S1060	Phosphorylation		Uniprot
S1062	Phosphorylation		Uniprot
S1064	Phosphorylation		Uniprot
T1070	Phosphorylation		Uniprot
S1075	Phosphorylation		Uniprot
S1099	Phosphorylation		Uniprot
K1128	Ubiquitination		Uniprot
S1174	Phosphorylation		Uniprot
S1192	Phosphorylation		Uniprot
K1200	Ubiquitination		Uniprot
S1202	Phosphorylation		Uniprot
K1207	Ubiquitination		Uniprot
K1213	Ubiquitination		Uniprot
Y1215	Phosphorylation		Uniprot
K1222	Ubiquitination		Uniprot
K1224	Ubiquitination		Uniprot
Y1296	Phosphorylation		Uniprot
K1341	Acetylation		Uniprot
K1341	Ubiquitination		Uniprot
S1344	Phosphorylation		Uniprot
S1363	Phosphorylation		Uniprot
K1405	Ubiquitination		Uniprot
K1414	Ubiquitination		Uniprot
K1425	Ubiquitination		Uniprot
S1465	Phosphorylation		Uniprot
K1497	Ubiquitination		Uniprot
Y1499	Phosphorylation		Uniprot
R1502	Methylation		Uniprot
Y1504	Phosphorylation		Uniprot
R1513	Methylation		Uniprot
Y1522	Phosphorylation		Uniprot
K1601	Ubiquitination		Uniprot
K1608	Ubiquitination		Uniprot
K1614	Ubiquitination		Uniprot
K1620	Ubiquitination		Uniprot
K1630	Ubiquitination		Uniprot
K1639	Ubiquitination		Uniprot
K1648	Ubiquitination		Uniprot
Y1664	Phosphorylation		Uniprot
S1689	Phosphorylation		Uniprot
K1722	Ubiquitination		Uniprot
K1739	Ubiquitination		Uniprot
K1749	Ubiquitination		Uniprot
T1768	Phosphorylation		Uniprot
S1769	Phosphorylation		Uniprot
S1771	Phosphorylation		Uniprot
T1772	Phosphorylation		Uniprot
S1803	Phosphorylation		Uniprot
T1805	Phosphorylation		Uniprot

研究背景

P52948_NUP98_HUMAN

功能:

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).

翻译修饰:

Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.

Proteolytically degraded after poliovirus (PV) infection; degradation is partial and NCP- and TPR-binding domains withstand degradation.

细胞定位:

Nucleus membrane>Peripheral membrane protein>Nucleoplasmic side. Nucleus>Nuclear pore complex. Nucleus>Nucleoplasm.
Note: Localized to the nucleoplasmic side of the nuclear pore complex (NPC), at or near the nucleoplasmic basket (PubMed:11839768). Dissociates from the dissasembled NPC structure early during prophase of mitosis (PubMed:12802065). Colocalized with NUP153 and TPR to the nuclear basket of NPC (PubMed:11839768). Colocalized with DHX9 in diffuse and discrete intranuclear foci (GLFG-body) (PubMed:11839768, PubMed:28221134). Remains localized to the nuclear membrane after poliovirus (PV) infection (PubMed:11106761).

亚基结构:

Part of the nuclear pore complex (NPC). Interacts directly with NUP96. Part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and NUP96; this complex plays a role in RNA export and in tethering NUP98 and NUP153 to the nucleus. Interacts with RAE1. Does not interact with TPR. Interacts with NUP88. Interacts directly with NUP88 and NUP214, subunits of the cytoplasmic filaments of the NPC (By similarity). Interacts (via N-terminus) with DHX9 (via DRBM, OB-fold and RGG domains); this interaction occurs in a RNA-dependent manner and stimulates DHX9-mediated ATPase activity.

(Microbial infection) Interacts with vesicular stomatitis virus protein M.

蛋白家族:

Contains G-L-F-G repeats. The FG repeat domains in Nup98 have a direct role in the transport.

Belongs to the nucleoporin GLFG family.

研究领域

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Viral > Influenza A.

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NUP98 Antibody - #DF8408

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