SRD5A2 Antibody - #DF8416

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产品: SRD5A2 抗体
货号: DF8416
描述: Rabbit polyclonal antibody to SRD5A2
应用: WB IHC
反应: Human
预测: Pig, Bovine, Horse, Sheep
分子量: 28 kDa; 28kD(Calculated).
蛋白号: P31213
RRID: AB_2841665

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%)
克隆:
Polyclonal
特异性:
SRD5A2 Antibody detects endogenous levels of total SRD5A2.
RRID:
AB_2841665
引用格式: Affinity Biosciences Cat# DF8416, RRID:AB_2841665.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3 oxo 5 alpha steroid 4 dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha SR2; 5 alpha-SR2; 5ART2; e II 5 alpha reductase; EC; MGC138457; Micropenis, included; S5A2_HUMAN; S5AR 2; SR type 2; SRD5A2; Steroid 5 alpha reductase 2; Steroid 5-alpha-reductase 2; Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2); Type II 5-alpha reductase;

抗原和靶标

免疫原:
Uniprot:

P31213(S5A2_HUMAN) >>Visit HPA database.

基因/基因ID:
SRD5A2(6716)
表达:
P31213 S5A2_HUMAN:

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

序列:
MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
78
Rabbit
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P31213 作为底物

Site PTM Type Enzyme
Y91 Phosphorylation
Y98 Phosphorylation
Y165 Phosphorylation
S177 Phosphorylation

研究背景

功能:

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

细胞定位:

Microsome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

蛋白家族:

Belongs to the steroid 5-alpha reductase family.

研究领域

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

文献引用

1). Risk subtyping and prognostic assessment of prostate cancer based on consensus genes. Communications Biology, 2022 (PubMed: 35293897) [IF=5.9]

Application: IHC    Species: Human    Sample: PCa tumor tissues

Fig. 4 Immunohistochemistry validation of SRD5A2 and ITGA11 expression in PCa tumor tissues. a Representative pictures showing the different protein levels of SRD5A2 in prostate cancer patients with different Gleason scores. Quantitative comparisons of H-scores were also performed in Gleason and pathological tumor stage subgroups. b Representative pictures showing the different protein levels of ITGA11 in prostate cancer patients with different Gleason scores. Quantitative comparison of staining density was also performed in Gleason and pathological tumor stage subgroups. Data are presented as the mean ± SD by t-test, bars represent mean values, error bars represent SD. Scale bars, 50 μm, 200 μm.

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