| 产品: | PIK3R4 抗体 |
| 货号: | DF8424 |
| 描述: | Rabbit polyclonal antibody to PIK3R4 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 153 kDa; 153kD(Calculated). |
| 蛋白号: | Q99570 |
| RRID: | AB_2841672 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8424, RRID:AB_2841672.
展开/折叠
MGC102700; P150; Phosphatidylinositol 3 kinase associated p150; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150; Phosphoinositide 3 kinase adaptor protein; Phosphoinositide 3 kinase regulatory subunit 4; Phosphoinositide 3 kinase regulatory subunit 4 p150; Phosphoinositide 3 kinase regulatory subunit polypeptide 4; Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150; Phosphoinositide 3-kinase adaptor protein; Phosphoinositide 3-kinase regulatory subunit 4; PI3 kinase p150 subunit; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; PI3R4_HUMAN; PIK3R 4; PIK3R4; VPS 15; VPS15;
抗原和靶标
- Q99570 PI3R4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99570 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| Y673 | Phosphorylation | Uniprot | |
| T680 | Phosphorylation | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| K772 | Ubiquitination | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| T780 | Phosphorylation | Uniprot | |
| K786 | Ubiquitination | Uniprot | |
| K791 | Ubiquitination | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| K816 | Ubiquitination | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| K850 | Ubiquitination | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| S861 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| K888 | Ubiquitination | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| S925 | Phosphorylation | Uniprot | |
| S926 | Phosphorylation | Uniprot | |
| T927 | Phosphorylation | Uniprot | |
| S931 | Phosphorylation | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| T942 | Phosphorylation | Uniprot | |
| K951 | Acetylation | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K962 | Ubiquitination | Uniprot | |
| K973 | Ubiquitination | Uniprot | |
| K993 | Ubiquitination | Uniprot | |
| K1018 | Ubiquitination | Uniprot | |
| Y1038 | Phosphorylation | Uniprot | |
| S1074 | Phosphorylation | Uniprot | |
| S1079 | Phosphorylation | Uniprot | |
| K1081 | Ubiquitination | Uniprot | |
| Y1272 | Phosphorylation | Uniprot | |
| S1282 | Phosphorylation | Uniprot | |
| T1283 | Phosphorylation | Uniprot | |
| S1287 | Phosphorylation | Uniprot | |
| S1289 | Phosphorylation | Uniprot | |
| Y1290 | Phosphorylation | Uniprot | |
| Y1291 | Phosphorylation | Uniprot | |
| K1293 | Ubiquitination | Uniprot | |
| K1307 | Ubiquitination | Uniprot | |
| K1309 | Ubiquitination | Uniprot | |
| T1316 | Phosphorylation | Uniprot |
研究背景
Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
Myristoylated.
Probably autophosphorylated.
Late endosome. Cytoplasmic vesicle>Autophagosome. Membrane>Lipid-anchor.
Note: As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Ubiquitously expressed.
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2. PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3C3/VPS34. Interacts with NRBF2.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
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