(13)
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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8427, RRID:AB_2841675.
展开/折叠
ESCRT I complex subunit TSG101; ESCRT-I complex subunit TSG101; TS101_HUMAN; TSG 10; TSG 101; TSG10; Tsg101; Tumor susceptibility gene 10; Tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; Tumor susceptibility protein; Tumor susceptibility protein isoform 3; VPS 23; VPS23;
抗原和靶标
- Q99816 TS101_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99816 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| K16 | Acetylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| Y63 | Phosphorylation | Uniprot | |
| K90 | Acetylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| K237 | Acetylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| Y390 | Phosphorylation | Uniprot |
研究背景
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes.
Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Late endosome membrane>Peripheral membrane protein. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody>Midbody ring. Nucleus.
Note: Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis.
Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry. Interacts with VPS37A, VPS37B and VPS37C. Interacts with DMAP1. Interacts with ubiquitin. Interacts with stathmin, GMCL and AATF (By similarity). Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry. Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding. Interacts with PDCD6. Interacts with LITAF. Interacts with MGRN1. Interacts with ARRDC1; recruits TSG101 to the plasma membrane.
(Microbial infection) Interacts with HIV-1 p6.
(Microbial infection) Interacts with human spumavirus Gag.
(Microbial infection) Interacts with HTLV-1 Gag.
(Microbial infection) Interacts with Ebola virus VP40.
(Microbial infection) Interacts with EIAV p9; the interaction has been shown in vitro.
(Microbial infection) Interacts with Lassa virus protein Z.
(Microbial infection) Interacts with hepatitis E virus protein ORF3.
The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
文献引用
Application: WB Species: Human Sample:
Application: WB Species: Mouse Sample: BM-MSCs cells
Application: WB Species: Human Sample: lung tissues
Application: WB Species: Rat Sample: CCSMCs
Application: WB Species: Human Sample:
Application: WB Species: human Sample: serum exosomes
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