产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8436, RRID:AB_2841682.
展开/折叠
Beta-hexosaminidase; Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidase; Bifunctional protein NCOAT; HEXC; Hyaluronidase in meningioma; KIAA0679; MEA5; Meningioma expressed antigen 5; Meningioma-expressed antigen 5; MGEA 5; Mgea5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; Nuclear cytoplasmic O GlcNAcase and acetyltransferase; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; OGA_HUMAN; Protein O-GlcNAcase;
抗原和靶标
- O60502 OGA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL
翻译修饰 - O60502 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K358 | Sumoylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| T370 | Phosphorylation | Uniprot | |
| S405 | O-Glycosylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| T709 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S712 | Phosphorylation | Uniprot | |
| K723 | Ubiquitination | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| K794 | Ubiquitination | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| K850 | Ubiquitination | Uniprot | |
| K858 | Ubiquitination | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| K885 | Ubiquitination | Uniprot | |
| K893 | Ubiquitination | Uniprot | |
| C896 | S-Nitrosylation | Uniprot | |
| K901 | Ubiquitination | Uniprot | |
| K907 | Ubiquitination | Uniprot | |
| S915 | Phosphorylation | Uniprot |
研究背景
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
Nucleus.
Cytoplasm.
Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
Monomer. Interacts with CLOCK (By similarity).
(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein Tax; this interaction increases Tax interacting partner CREB1 O-GlcNAcylation.
Belongs to the glycosyl hydrolase 84 family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
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