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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8437, RRID:AB_2841683.
展开/折叠
CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1;
抗原和靶标
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
- P50749 RASF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYSHQTSLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISWGLRRPIRLQMQDDNERIRPPPSSSSWHSGCNLGAQGTTLKPLTVPKVQISEVDAPPEGDQMPSSTDSRGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTVLRLMIRQRLEEIAETPATI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50749 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| R142 | Methylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| R144 | Methylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| R151 | Methylation | Uniprot | |
| R165 | Methylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot |
研究背景
Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Phosphorylated by STK3/MST2 and STK4/MST1.
Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
Interacts directly with activated KRAS in a GTP-dependent manner. Interacts (via SARAH domain) with STK3/MST2 AND STK4/MST1.
研究领域
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
文献引用
Application: WB Species: human Sample:
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