产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8517, RRID:AB_2841722.
展开/折叠
Alkaline phosphodiesterase I; ARHR2; B10; CD203c; CD203c antigen; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); E NPP 3; E-NPP 3; Ectonucleotide pyrophosphatase/phosphodiesterase 3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; ENPP3; ENPP3_HUMAN; gp130RB13 6; gp130RB136; M6S1; NPP1; NPP3; NPPase; NPPS; Nucleotide pyrophosphatase; PC-1; PCA1; PD Ibeta; PD-Ibeta; PDNP1; PDNP3; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase i/nucleotide pyrophosphatase beta;
抗原和靶标
Detected on bile ducts in liver, and in blood serum (at protein level) (PubMed:15072822). Detected in prostate and uterus (PubMed:9344668). Detected on basophils, but not neutrophils (PubMed:11342463).
- O14638 ENPP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESTLTLATEQPVKKNTLKKYKIACIVLLALLVIMSLGLGLGLGLRKLEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14638 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T205 | Phosphorylation | O14638 (ENPP3) | Uniprot |
| N236 | N-Glycosylation | Uniprot | |
| N279 | N-Glycosylation | Uniprot | |
| N290 | N-Glycosylation | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| N426 | N-Glycosylation | Uniprot | |
| N533 | N-Glycosylation | Uniprot | |
| N582 | N-Glycosylation | Uniprot | |
| N594 | N-Glycosylation | Uniprot | |
| N687 | N-Glycosylation | Uniprot | |
| T689 | Phosphorylation | Uniprot | |
| Y694 | Phosphorylation | Uniprot | |
| N699 | N-Glycosylation | Uniprot | |
| N789 | N-Glycosylation | Uniprot | |
| T846 | Phosphorylation | Uniprot | |
| Y851 | Phosphorylation | Uniprot |
研究背景
Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has also alkaline phosphodiesterase activity.
N-glycosylated. N-glycosylation is necessary for normal transport to the cell membrane, but is not the apical targeting signal.
Cell membrane>Single-pass type II membrane protein. Apical cell membrane>Single-pass type II membrane protein. Secreted.
Note: Detected at the cell surface of basophils (PubMed:11342463). Detected at the apical plasma membrane of bile duct cells (PubMed:15072822). Located to the apical surface in intestinal and kidney epithelial cells. Secreted in serum, and in lumen of epithelial cells.
Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.
Monomer and homodimer.
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Riboflavin metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Pantothenate and CoA biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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