产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8539, RRID:AB_2841743.
展开/折叠
CED 12; Ced 12 homolog 1; Ced 12 homolog; CED-12; CED12; Ced12 homolog 1; Ced12 homolog; ELMO 1; ELMO-1; Elmo1; ELMO1_HUMAN; Engulfment and cell motility 1; Engulfment and cell motility protein 1; KIAA0281; MGC126406; Protein ced-12 homolog;
抗原和靶标
- Q92556 ELMO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPPPADIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFALQHADSSNFYITEKNRNEIKNGTILRLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERYQKLQKIMKPCFGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQRSLAILESMVLNSHDLYQKVAQEITIGQLIPHLQGSDQEIQTYTIAVINALFLKAPDERRQEMANILAQKQLRSIILTHVIRAQRAINNEMAHQLYVLQVLTFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKSMYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIELTKMLCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMRALTTKPSSLDQFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGTCFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGALKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLDLENIQIPDAPPPIPKEPSNYDFVYDCN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92556 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| Y18 | Phosphorylation | P08631 (HCK) | Uniprot |
| K20 | Ubiquitination | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K100 | Acetylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K105 | Acetylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | P08631 (HCK) | Uniprot |
| K269 | Ubiquitination | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| R328 | Methylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| Y356 | Phosphorylation | Uniprot | |
| Y395 | Phosphorylation | P08631 (HCK) | Uniprot |
| K408 | Acetylation | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| K485 | Acetylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| Y511 | Phosphorylation | P08631 (HCK) | Uniprot |
| T512 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K549 | Ubiquitination | Uniprot | |
| Y576 | Phosphorylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| K635 | Acetylation | Uniprot | |
| K695 | Ubiquitination | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| Y720 | Phosphorylation | P08631 (HCK) , P12931 (SRC) | Uniprot |
| Y724 | Phosphorylation | P12931 (SRC) | Uniprot |
研究背景
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Phosphorylated by HCK.
Cytoplasm. Cell membrane.
Note: Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
Widely expressed, with a higher expression in the spleen and placenta.
Interacts with ADGRB1 (By similarity). Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Part of a complex with DOCK1 and RAC1. Part of a complex with DOCK1 and CRK isoform CRK-II. Interacts with PLEKHG6. Interacts with HCK (via SH3 domain). Interacts with ADGRB3.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
限制条款
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