GCLC Antibody | Affinity Biosciences LTD|亲科生物官网

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IF/ICC
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Western blot analysis of extracts from various samples, using GCLC Antibody.

Western blot analysis of extracts from various samples, using GCSc-γ Antibody.

DF8550 staining Hela cells by IF/ICC.

Figure 3: Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells.

Figure 9.

FIGURE 7 |Effects of different doses of echinacoside (ECH) on the expressions of nuclear factor E2 p45‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and γ‐glutamyl cysteine synthetase (γ‐GCS) in the mice exposed to hypobaric hypoxia in protein level.

Figure 5.

Fig.

产品:	GCLC 抗体
货号:	DF8550
描述:	Rabbit polyclonal antibody to GCLC
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat, Pig
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	72 kDa; 73kD(Calculated).
蛋白号:	P48506
RRID:	AB_2841754

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Pig

预测:

Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

GCLC Antibody detects endogenous levels of total GCLC.

RRID:

AB_2841754
引用格式: Affinity Biosciences Cat# DF8550, RRID:AB_2841754.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

EC 6.3.2.2; Gamma ECS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; Gclc; GCS; GCS heavy chain; GLCL; GLCLC; Glutamate cysteine ligase catalytic subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN;

抗原和靶标

免疫原:

Uniprot:

P48506(GSH1_HUMAN) >>Visit HPA database.

基因/基因ID:

GCLC(2729)

序列:

P48506 GSH1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48506 作为底物

P48506

Site	PTM Type	Source
M1	Acetylation	Uniprot
S5	Phosphorylation	Uniprot
S8	Phosphorylation	Uniprot
T15	Phosphorylation	Uniprot
K16	Acetylation	Uniprot
Y53	Phosphorylation	Uniprot
S70	Phosphorylation	Uniprot
K73	Ubiquitination	Uniprot
K81	Ubiquitination	Uniprot
K161	Ubiquitination	Uniprot
K209	Ubiquitination	Uniprot
K211	Ubiquitination	Uniprot
S215	Phosphorylation	Uniprot
T220	Phosphorylation	Uniprot
T222	Phosphorylation	Uniprot
K318	Ubiquitination	Uniprot
Y322	Phosphorylation	Uniprot
S334	Phosphorylation	Uniprot
K338	Ubiquitination	Uniprot
K342	Ubiquitination	Uniprot
Y343	Phosphorylation	Uniprot
T381	Phosphorylation	Uniprot
S395	Phosphorylation	Uniprot
K412	Ubiquitination	Uniprot
S464	Phosphorylation	Uniprot
K465	Ubiquitination	Uniprot
K492	Acetylation	Uniprot
Y587	Phosphorylation	Uniprot
S621	Phosphorylation	Uniprot

研究背景

P48506_GSH1_HUMAN

亚基结构:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

蛋白家族:

Belongs to the glutamate--cysteine ligase type 3 family.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. FOOD RESEARCH INTERNATIONAL, 2021 (PubMed: 33509464) [IF=8.1]

2). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE, 2022 (PubMed: 36150346) [IF=7.9]

3). Echinacoside alleviates hypobaric hypoxia-induced memory impairment in C57 mice. PHYTOTHERAPY RESEARCH, 2019 (PubMed: 30768741) [IF=7.2]

Application: WB Species: mouse Sample: Hippocampi

FIGURE 7 |Effects of different doses of echinacoside (ECH) on the expressions of nuclear factor E2 p45‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and γ‐glutamyl cysteine synthetase (γ‐GCS) in the mice exposed to hypobaric hypoxia in protein level. Hippocampi of the mice were collected, and the protein levels of Nrf2 (cytoplasmic and nuclear), HO‐1, NQO1, and γ‐GCS were evaluated by Western blot. *p < 0.05, **p < 0.01 versus control; #p < 0.05, ##p < 0.01 versus hypoxia (HYP)

4). Therapeutic role of D-pinitol on experimental colitis via activating Nrf2/ARE and PPAR-γ/NF-κB signaling pathways. Food & Function, 2021 (PubMed: 33625409) [IF=6.1]

5). Taraxasterol protects against acetaminophen-induced hepatotoxicity by reducing liver inflammatory response and ameliorating oxidative stress in mice. International immunopharmacology, 2024 (PubMed: 38943970) [IF=5.6]

6). Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug. International immunopharmacology, 2021 (PubMed: 34628270) [IF=5.6]

Application: WB Species: Rat Sample: liver tissues

Fig. 6. Curc-mPEG454 up-regulates GCLC and GCLM for GSH de novo synthesis. (A) Heatmap represents gene expression of Glutathione metabolism. Gene related to glutathione synthesis and metabolism are indicated. (B) Paired display the gene expression by RNA-seq and relative mRNA expression by qRT-PCR from glutathione synthesis and metabolism. Gene expression level was measured in FPKM value. The mRNA levels were normalized to GAPDH, and subsequently represents as fold change relative to control group. (C) The protein levels of NRF2, GCLC and GCLM. (D) Curc-mPEG454-100 mg significantly restores GSH level during liver fibrosis. Contents of GSH in liver tissues were detected at the 6th week. Data are expressed as mean ± SEM. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with normal group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with CCl4-induced model group.

7). The TβR II-targeted aptamer S58 prevents fibrosis after glaucoma filtration surgery. Aging-US, 2020 (PubMed: 32452828) [IF=5.2]

Application: WB Species: human Sample: HConFs

Figure 5. | S58 promotes antioxidant defense of TGF-β2-induced HConFs. (A) Analysis of antioxidant capacity of cells at day 14 after GFS. (B) Intracellular ROS variation, and (C) mitochondrial superoxide variation were examined by flow cytometry. (D) Antioxidant protein SOD1/2, γ-GCS and CAT levels analyzed by western blotting in TGF-β2-treated HConFs in the presence or absence of S58 (20 nM). (E) Relative antioxidant gene levels in HConFs preconditioned with TGF-β2 in the presence or absence of S58 (20 nM) for 12h. All data indicate the mean ± SD, n=3. *p < 0.05, **p < 0.01, ***p < 0.001.

8). Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FOOD AND CHEMICAL TOXICOLOGY, 2017 (PubMed: 28137608) [IF=4.3]

Application: WB Species: rat Sample: PC12 cells

9). Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. FOOD AND CHEMICAL TOXICOLOGY, 2019 (PubMed: 30423402) [IF=4.3]

Application: WB Species: mouse Sample: liver

Figure 9. |Differential effects of THC, OHC and CUR on NQO1 and HO-1 expressions in liver. (A)Densitometry analysis of mRNA level; (B) Representative protein expression bands; (C) Densitometry analysis of protein level. Data are presented as the mean ± SD (n = 3). ##p < 0.01 versus control group. *p < 0.05, **p < 0.01 versus APAP-treated group. ∆p < 0.05, ∆∆p < 0.01 versus treatment of CUR group.

10). Circ-Sirt1 inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. AUTOIMMUNITY, 2021 (PubMed: 34431434) [IF=3.5]

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GCLC Antibody - #DF8550

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