GnRH I Antibody - #DF8553

Fig. 4. | N-linked glycosylation is involved in the process of Oatp1a5 transporting MC-LR. GT1-7 cells were pretreated with N-linked glycosylation inhibitor tunicamycin at a concentration of 0.01 μg/mL for 1 h, and followed by the treatment with MC-LR at a concentration of 500 nM for 24 h. (A) The presence of MC-LR in the cells was measured by immunofluorescence assay (scale bar = 50 μm). (B) Intracellular uptake of MC-LR by the transfected cells were detected by western blotting using an anti-MC-LR antibody. (C) The expression of GnRH was examined by western blotting.

Fig. 2. MC-LR induced apoptosis of GnRH neurons. (A) Apoptotic cells in the hypothalamus of mice were detected by TUNEL test. The apoptotic cells with brown stained nuclei were stained by DAB, and apoptotic index was calculated based on the percentage of TUNEL positive cells in an image (scale bar = 50 µm, n = 6 mice/group, **P < 0.01 vs. the control). (B) A confocal fluorescence microscope was used to detect colocalization of Alexa Fluor 594-labled GPR54 (red), Alexa Fluor 488-labled active-caspase3 (green) and DAPI (blue) (scale bar = 50 µm, n = 3). The marker protein of GnRH neurons is GPR54.(For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)