GRO gamma Antibody - #DF8554

Figure 4 Characterization of myeloid cells in the seminoma testes. (A) t-SNE plots for seven distinct myeloid cell subclusters. (B) Dot plot showing the expression of the top five marker genes in each myeloid cell subcluster. (C) Violin plots showing the expression level of selected marker genes across the clusters. (D) Multiplex immunofluorescence staining for CD1C, CLEC9A, LAMP3, and DAPI in seminoma testes (scale bar: 100 μm). (E) Heatmap of the expression of the maturation, regulatory, migration, and Toll-like receptor (TLR)-related marker genes in each cluster. (F) Bubble plots showing the probability of ligand–receptor pairs between tumor cell cluster 1/3 and three types of dendritic cell subclusters. (G) Heatmap of the expression of the M1, M2, angiogenesis, and phagocytosis-related marker genes in two types of macrophages. (H) Multiplex immunofluorescence staining for SELENOP, CXCL3, and DAPI in seminoma testes (scale bar: 100 μm).

Fig. 6. Regulatory effects of integrin β6 (ITGB6) on CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 expression levels in sweat gland cells. Sweat gland cells extracted from patients with primary palmar hyperhidrosis were treated with blank control or transfected with overexpression negative control (OE NC) or ITGB6 OE. The CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 (A) mRNA (ITGB6 OE compared to control, Tukey’s honest significant difference (HSD), p < 0.001, all), and (B,C) protein expression (ITGB6 OE compared to control, CXCL3, CXCL5, and CXCL11: Tukey’s HSD, all p < 0.001; CXCL10: Tukey’s HSD, p = 0.004; WNT2: Tukey’s HSD, p = 0.027). The data are presented as mean ± 95% confidence interval (95% CI)