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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8561, RRID:AB_2841765.
展开/折叠
D2HGA2; ICD-M; IDH; IDH2; IDHM; IDHP_HUMAN; IDP; IDPM; Isocitrate dehydrogenase [NADP], mitochondrial; Isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase;
抗原和靶标
- P48735 IDHP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPVVEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P48735 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K48 | Acetylation | Uniprot | |
| K67 | Acetylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K80 | Acetylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| T117 | Phosphorylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| K155 | Acetylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| R188 | Methylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K242 | Acetylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K263 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K282 | Acetylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| C308 | S-Nitrosylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| K384 | Acetylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K413 | Acetylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K442 | Acetylation | Uniprot | |
| K442 | Ubiquitination | Uniprot |
研究背景
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
Mitochondrion.
Homodimer.
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
研究领域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
文献引用
Application: WB Species: Human Sample: HepG2 cells
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