RNase III Drosha Antibody - #DF8601

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产品: RNase III Drosha 抗体
货号: DF8601
描述: Rabbit polyclonal antibody to RNase III Drosha
应用: WB
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 159 kDa; 159kD(Calculated).
蛋白号: Q9NRR4
RRID: AB_2841805

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
RNase III Drosha Antibody detects endogenous levels of total RNase III Drosha.
RRID:
AB_2841805
引用格式: Affinity Biosciences Cat# DF8601, RRID:AB_2841805.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DROSHA; Drosha double stranded RNA specific endoribonuclease; Drosha ribonuclease type III; Etohi2; HSA242976; Nuclear RNase III Drosha; p241; Protein Drosha; Putative protein p241 which interacts with transcription factor Sp1; Putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; Ribonuclease III nuclear; Ribonuclease type III nuclear; RibonucleaseIII; RN 3; RN3; RNase 3; RNase III; RNase3; RNASE3L; RNaseIII; RNASEN; RNC_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q9NRR4(RNC_HUMAN) >>Visit HPA database.

基因/基因ID:
DROSHA(29102)
表达:
Q9NRR4 RNC_HUMAN:

Ubiquitous.

序列:
MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQGTFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRSYERSRERERERHRHRDNRRSPSLERSYKKEYKRSGRSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEIVNHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDETEDIKK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NRR4 作为底物

Site PTM Type Enzyme
S221 Phosphorylation Q16539 (MAPK14)
Y228 Phosphorylation
S237 Phosphorylation
S255 Phosphorylation Q16539 (MAPK14)
S267 Phosphorylation
Y269 Phosphorylation
R271 Methylation
T274 Phosphorylation Q16539 (MAPK14)
S276 Phosphorylation
S300 Phosphorylation Q16539 (MAPK14) , P49841 (GSK3B)
S302 Phosphorylation P49841 (GSK3B)
S317 Phosphorylation
S355 Phosphorylation Q16539 (MAPK14)
S357 Phosphorylation
S373 Phosphorylation
K382 Ubiquitination
K388 Sumoylation
K409 Sumoylation
K409 Ubiquitination
K447 Ubiquitination
S491 Phosphorylation
S698 Phosphorylation
Y705 Phosphorylation
K738 Ubiquitination
T747 Phosphorylation
K748 Ubiquitination
K792 Ubiquitination
K795 Ubiquitination
S796 Phosphorylation
S807 Phosphorylation
K809 Ubiquitination
K827 Ubiquitination
K831 Ubiquitination
K1207 Ubiquitination
K1250 Ubiquitination
K1262 Ubiquitination
K1277 Ubiquitination
K1285 Ubiquitination
S1293 Phosphorylation
K1313 Ubiquitination
K1331 Ubiquitination
K1341 Ubiquitination
K1353 Ubiquitination
K1373 Ubiquitination

研究背景

功能:

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.

细胞定位:

Nucleus. Nucleus>Nucleolus.
Note: A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with SP1 and SNIP1. Interacts with SRRT/ARS2 (By similarity). Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner (By similarity).

蛋白家族:

The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.

Belongs to the ribonuclease III family.

研究领域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

文献引用

1). Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus. Cells, 2022 (PubMed: 36359855) [IF=6.0]

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