SEMA3F Antibody - #DF8611

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产品: SEMA3F 抗体
货号: DF8611
描述: Rabbit polyclonal antibody to SEMA3F
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 88 kDa; 88kD(Calculated).
蛋白号: Q13275
RRID: AB_2841815

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
SEMA3F Antibody detects endogenous levels of total SEMA3F.
RRID:
AB_2841815
引用格式: Affinity Biosciences Cat# DF8611, RRID:AB_2841815.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

SEM3F_HUMAN; sema domain immunoglobulin domain Ig short basic domain secreted 3F; sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F; Sema III/F; Sema IV; SEMA-IV; SEMA3F; SEMA4; SemaIII/F; SemaIV; SEMAK; Semaphorin IV; Semaphorin-3F; Semaphorin3F; SemaphorinIV;

抗原和靶标

免疫原:
Uniprot:

Q13275(SEM3F_HUMAN) >>Visit HPA database.

基因/基因ID:
SEMA3F(6405)
表达:
Q13275 SEM3F_HUMAN:

Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.

序列:
MLVAGLLLWASLLTGAWPSFPTQDHLPATPRVRLSFKELKATGTAHFFNFLLNTTDYRILLKDEDHDRMYVGSKDYVLSLDLHDINREPLIIHWAASPQRIEECVLSGKDVNGECGNFVRLIQPWNRTHLYVCGTGAYNPMCTYVNRGRRAQATPWTQTQAVRGRGSRATDGALRPMPTAPRQDYIFYLEPERLESGKGKCPYDPKLDTASALINEELYAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSAERNDDKLYFFFRERSAEAPQSPAVYARIGRICLNDDGGHCCLVNKWSTFLKARLVCSVPGEDGIETHFDELQDVFVQQTQDVRNPVIYAVFTSSGSVFRGSAVCVYSMADIRMVFNGPFAHKEGPNYQWMPFSGKMPYPRPGTCPGGTFTPSMKSTKDYPDEVINFMRSHPLMYQAVYPLQRRPLVVRTGAPYRLTTIAVDQVDAADGRYEVLFLGTDRGTVQKVIVLPKDDQELEELMLEEVEVFKDPAPVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCLARDPYCAWDGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAVESVQYGVAGSAAFLECQPRSPQATVKWLFQRDPGDRRREIRAEDRFLRTEQGLLLRALQLSDRGLYSCTATENNFKHVVTRVQLHVLGRDAVHAALFPPLSMSAPPPPGAGPPTPPYQELAQLLAQPEVGLIHQYCQGYWRHVPPSPREAPGAPRSPEPQDQKKPRNRRHHPPDT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
92
Rabbit
83
Chicken
43
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13275 作为底物

Site PTM Type Enzyme
Y131 Phosphorylation
Y144 Phosphorylation
K198 Acetylation
K200 Acetylation
K425 Acetylation
S437 Phosphorylation
Y442 Phosphorylation
Y478 Phosphorylation
T485 Phosphorylation
S766 Phosphorylation

研究背景

功能:

May play a role in cell motility and cell adhesion.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.

蛋白家族:

Belongs to the semaphorin family.

研究领域

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35265682) [IF=2.8]

Application: WB    Species: Rat    Sample: hippocampus tissue

Figure 7 Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, VIPR1, and FAM120A, SEMA3F determined by western blot. *p < 0.05, **p < 0.01, ***p < 0.001, and ns, non-significant.

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