ZO-2 Antibody - #DF8632

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产品: ZO-2 抗体
货号: DF8632
描述: Rabbit polyclonal antibody to ZO-2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 133 kDa; 134kD(Calculated).
蛋白号: Q9UDY2
RRID: AB_2841836

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(92%)
克隆:
Polyclonal
特异性:
ZO-2 Antibody detects endogenous levels of total ZO-2.
RRID:
AB_2841836
引用格式: Affinity Biosciences Cat# DF8632, RRID:AB_2841836.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); MGC26306; PFIC4; Tight junction protein 2; Tight junction protein ZO 2; Tight junction protein ZO-2; TJP2; X104; ZO 2; ZO-2; ZO2; ZO2_HUMAN; Zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2;

抗原和靶标

免疫原:
Uniprot:

Q9UDY2(ZO2_HUMAN) >>Visit HPA database.

基因/基因ID:
TJP2(9414)
表达:
Q9UDY2 ZO2_HUMAN:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

序列:
MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTATINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASSDQLRDNSPPPAFKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGNETPGASTKGYPPPVAAKPTFGRSILKPSTPIPPQEGEEVGESSEEQDNAPKSVLGKVKIFEKMDHKARLQRMQELQEAQNARIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Horse
92
Dog
92
Chicken
92
Rabbit
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UDY2 作为底物

Site PTM Type Enzyme
S16 Phosphorylation
T91 Phosphorylation
S130 Phosphorylation
S150 Phosphorylation
S153 Phosphorylation
S156 Phosphorylation
S163 Phosphorylation
S168 Phosphorylation
S170 Phosphorylation
S174 Phosphorylation
S186 Phosphorylation
S192 Phosphorylation
S196 Phosphorylation
S200 Phosphorylation
T212 Phosphorylation
S216 Phosphorylation
S220 Phosphorylation
S232 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
Y249 Phosphorylation
Y253 Phosphorylation
Y257 Phosphorylation
Y261 Phosphorylation
Y265 Phosphorylation
S266 Phosphorylation
Y269 Phosphorylation
S279 Phosphorylation
S284 Phosphorylation
S286 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
S296 Phosphorylation
Y319 Phosphorylation
S325 Phosphorylation
T333 Phosphorylation
T335 Phosphorylation
T339 Phosphorylation
S385 Phosphorylation
S394 Phosphorylation
S398 Phosphorylation
S400 Phosphorylation
S406 Phosphorylation
S413 Phosphorylation
S415 Phosphorylation
Y423 Phosphorylation
S424 Phosphorylation
Y426 Phosphorylation
Y428 Phosphorylation
S430 Phosphorylation
S431 Phosphorylation
S432 Phosphorylation
S440 Phosphorylation
S441 Phosphorylation
T445 Phosphorylation
S450 Phosphorylation
T455 Phosphorylation
T457 Phosphorylation
K460 Methylation
S461 Phosphorylation
T462 Phosphorylation
T468 Phosphorylation
K475 Ubiquitination
Y479 Phosphorylation
T494 Phosphorylation
S499 Phosphorylation
Y506 Phosphorylation
T510 Phosphorylation
S520 Phosphorylation
Y574 Phosphorylation
T585 Phosphorylation
S590 Phosphorylation
S606 Phosphorylation
Y638 Phosphorylation
S702 Phosphorylation
T707 Phosphorylation
S711 Phosphorylation
S713 Phosphorylation
K730 Methylation
T760 Phosphorylation
S767 Phosphorylation
K769 Ubiquitination
S770 Phosphorylation
T771 Phosphorylation
T778 Phosphorylation
T831 Phosphorylation
S832 Phosphorylation
S836 Phosphorylation
S886 Phosphorylation
S902 Phosphorylation
Y903 Phosphorylation
T905 Phosphorylation
Y911 Phosphorylation
S913 Phosphorylation
S916 Phosphorylation
S920 Phosphorylation
T925 Phosphorylation
Y932 Phosphorylation
T933 Phosphorylation
S953 Phosphorylation
S961 Phosphorylation
S966 Phosphorylation
S978 Phosphorylation
S979 Phosphorylation
S986 Phosphorylation
S1006 Phosphorylation
Y1007 Phosphorylation
S1012 Phosphorylation
Y1013 Phosphorylation
Y1015 Phosphorylation
K1016 Ubiquitination
T1027 Phosphorylation
S1031 Phosphorylation
S1048 Phosphorylation
S1053 Phosphorylation
T1054 Phosphorylation
S1067 Phosphorylation
S1068 Phosphorylation
Y1118 Phosphorylation
T1124 Phosphorylation
T1131 Phosphorylation
T1135 Phosphorylation
S1137 Phosphorylation
S1147 Phosphorylation
Y1150 Phosphorylation
S1156 Phosphorylation
Y1157 Phosphorylation
S1159 Phosphorylation
Y1166 Phosphorylation
S1171 Phosphorylation
S1174 Phosphorylation
R1176 Methylation
Y1178 Phosphorylation
Y1179 Phosphorylation
S1182 Phosphorylation

研究背景

功能:

Plays a role in tight junctions and adherens junctions.

细胞定位:

Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

亚基结构:

Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus. Interacts with SCRIB. Interacts with USP53 (via the C-terminal region) (By similarity).

蛋白家族:

Belongs to the MAGUK family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

文献引用

1). Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 31863867) [IF=9.3]

Application: WB    Species: Mice    Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.
2). A novel nano material for anti-cerebral ischemia: Preparation and Application of Borneol Angelica Polysaccharide Liposomes. JOURNAL OF LIPOSOME RESEARCH, 2023 (PubMed: 35875973) [IF=4.4]
3). Preparation of Borneol Angelica Polysaccharide Liposomes and Study Evaluation of Its Anti-Cerebral Ischemia-Reperfusion Inflammatory Reaction. Research Square, 2021

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