| 产品: | APC8 抗体 |
| 货号: | DF8637 |
| 描述: | Rabbit polyclonal antibody to APC8 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 68 kDa; 69kD(Calculated). |
| 蛋白号: | Q9UJX2 |
| RRID: | AB_2841841 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8637, RRID:AB_2841841.
展开/折叠
ANAPC8; Anaphase promoting complex subunit 8; Anaphase-promoting complex subunit 8; Apc 8; APC8; Cdc 23; CDC23; CDC23_HUMAN; cell division cycle 23; Cell division cycle 23 homolog; Cell division cycle protein 23; Cell division cycle protein 23 homolog; Cut23; Cyclosome subunit 8;
抗原和靶标
- Q9UJX2 CDC23_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UJX2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K147 | Sumoylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| Y340 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| K467 | Acetylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| K522 | Methylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| T542 | Phosphorylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| T562 | Phosphorylation | P53350 (PLK1) , P06493 (CDK1) | Uniprot |
| T564 | Phosphorylation | Uniprot | |
| T565 | Phosphorylation | P53350 (PLK1) , P06493 (CDK1) | Uniprot |
| S576 | Phosphorylation | Uniprot | |
| S578 | Phosphorylation | Uniprot | |
| T582 | Phosphorylation | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| T596 | Phosphorylation | Uniprot |
研究背景
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.
Belongs to the APC8/CDC23 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
限制条款
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