产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8660, RRID:AB_2841864.
展开/折叠
ARF GAP GIT1; ARF GTPase activating protein GIT1; ARF GTPase-activating protein GIT1; CAT 1; CAT-1; CaT1; Cool associated and tyrosine phosphorylated protein 1; Cool-associated and tyrosine-phosphorylated protein 1; G protein coupled receptor kinase interacting ArfGAP 1; G protein coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GIT1_HUMAN; GRK interacting protein 1; GRK-interacting protein 1;
抗原和靶标
- Q9Y2X7 GIT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y2X7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S36 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | O94806 (PRKD3) | Uniprot |
| K49 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| Y284 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Q05397 (PTK2) , P12931 (SRC) | Uniprot |
| K358 | Ubiquitination | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| Y383 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| Y510 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| K517 | Methylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| T537 | Phosphorylation | Uniprot | |
| Y545 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| Y554 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| T601 | Phosphorylation | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| C674 | S-Nitrosylation | Uniprot | |
| K677 | Acetylation | Uniprot | |
| S700 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| T753 | Phosphorylation | Uniprot |
研究背景
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).
Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).
Cytoplasm.
Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1 (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. Interacts with SCRIB. Interacts (via N- and C-terminus) with ENTR1 (via N-terminus); this interaction is direct. Found in a complex with ENTR1, PTPN13 and GIT1. Interacts with YWHAZ (By similarity).
The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
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