IP6K2 Antibody - #DF8669

Fig. 4. Molecular and microstructural analysis of the 4-week-old R6/1-Foxp2 mice striatum. (a) Volcano plot of downregulated (red) and upregulated proteins (blue) of R6/1-GFP striatum respect to WT-GFP mice. (b) Volcano plot of downregulated (red) and upregulated proteins (blue) of R6/1-Foxp2 mice striatum respect to WT-GFP mice. (c) Heat map of clusters of downregulated (red) and upregulated proteins (blue) of R6/1-GFP and R6/1-Foxp2 mice striatum respect to WT-GFP mice. Cutoffs of ±0.5-fold change and p < 0.05. (n = 4 mice / group). (d) Golgi-Cox-stained dendrites from medium spiny neurons in the dorsal striatum. Scale bar, 7 μm. (e) Quantification of spine density in dendrites as in d. Two-way ANOVA, group effect: F(1,302) = 9.678; p = 0.0020; treatment effect: F(1,302) = 9.728; p = 0.0020; interaction effect: F(1,302) = 14.65; p = 0.0002. Sidak's post hoc test (15 dendrites per animal / 4–5 mice / group). Densitometric quantification of striatal Ip6k2 (f), Arhgap4 (g) and Tor1A (h) protein levels in WT-GFP, R6/1-GFP, WT-Foxp2 and R6/1-Foxp2 mice. As a loading control α-tubulin was used in f-h. Data are means ± SEM. WT-GFP (n = 7), R6/1 GFP (n = 7); WT Foxp2 (n = 7); R6/1 Foxp2 (n = 7). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)