产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8725, RRID:AB_2841929.
展开/折叠
bHLHa17; Class A basic helix-loop-helix protein 17; OTTHUMP00000009563; OTTHUMP00000009564; SCL; STEM CELL LEUKEMIA HEMATOPOIETIC TRANSCRIPTION FACTOR; Stem cell protein; T cell acute lymphocytic leukemia 1; T cell acute lymphocytic leukemia 1 protein; T cell acute lymphocytic leukemia 1 protein; T cell leukemia/lymphoma 5 protein; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; Tal 1; Tal 1 product; TAL 1 protein; TAL-1; tal1; TAL1_HUMAN; TCL 5; TCL5;
抗原和靶标
- P17542 TAL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTERPPSEAARSDPQLEGRDAAEASMAPPHLVLLNGVAKETSRAAAAEPPVIELGARGGPGGGPAGGGGAARDLKGRDAATAEARHRVPTTELCRPPGPAPAPAPASVTAELPGDGRMVQLSPPALAAPAAPGRALLYSLSQPLASLGSGFFGEPDAFPMFTTNNRVKRRPSPYEMEITDGPHTKVVRRIFTNSRERWRQQNVNGAFAELRKLIPTHPPDKKLSKNEILRLAMKYINFLAKLLNDQEEEGTQRAKTGKDPVVGAGGGGGGGGGGAPPDDLLQDVLSPNSSCGSSLDGAASPDSYTEEPAPKHTARSLHPAMLPAADGAGPR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17542 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T91 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | P27361 (MAPK3) , Q02750 (MAP2K1) , P17612 (PRKACA) | Uniprot |
| Y138 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K212 | Acetylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K222 | Acetylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K225 | Acetylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S316 | Phosphorylation | Uniprot |
研究背景
Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation (By similarity).
Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia (By similarity).
Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells (By similarity).
Nucleus.
Leukemic stem cell.
Efficient DNA binding requires dimerization with another bHLH protein. Forms heterodimers with TCF3. Binds to the LIM domain containing protein LMO2 and to DRG1. Can assemble in a complex with LDB1 and LMO2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with SBNO2; this interaction inhibits TAL1 occupancy of the DCSTAMP promoter, leading to the activation of the DCSTAMP promoter by the transcription factor MITF.
The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.
限制条款
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