产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8746, RRID:AB_2841950.
展开/折叠
130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein; 130 kDa phosphatidylinositol 4; 5-biphosphate-dependent ARF1 GTPase-activating protein; ADP ribosylation factor directed GTPase activating protein 1; ADP-ribosylation factor-directed GTPase-activating protein 1; AMAP 1; AMAP1; ANK repeat and PH domain-containing protein 1; ARF GTPase activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain; ASAP 1; ASAP1; ASAP1_HUMAN; Centaurin beta 4; CentB4; DDEF 1; DDEF1 protein; DEF-1; Development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; KIAA1249; PAG 2; PAG2; PAP; PIP2 dependent ARF1 GAP; PIP2-dependent ARF1 GAP; ZG 14P; ZG14P;
抗原和靶标
- Q9ULH1 ASAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKATIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD
翻译修饰 - Q9ULH1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| K182 | Acetylation | Uniprot | |
| K184 | Acetylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| K555 | Acetylation | Uniprot | |
| K563 | Acetylation | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S733 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| T758 | Phosphorylation | Uniprot | |
| Y767 | Phosphorylation | Q14289 (PTK2B) | Uniprot |
| K800 | Ubiquitination | Uniprot | |
| S816 | Phosphorylation | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| T837 | Phosphorylation | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| S877 | Phosphorylation | Uniprot | |
| S881 | Phosphorylation | Uniprot | |
| K905 | Acetylation | Uniprot | |
| S910 | Phosphorylation | Uniprot | |
| T915 | Phosphorylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| S925 | Phosphorylation | Uniprot | |
| S1008 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| S1032 | Phosphorylation | Uniprot | |
| S1041 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot | |
| T1048 | Phosphorylation | Uniprot | |
| T1054 | Phosphorylation | Uniprot | |
| T1097 | Phosphorylation | Uniprot | |
| S1122 | Phosphorylation | Uniprot | |
| S1128 | Phosphorylation | Uniprot |
研究背景
Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
Phosphorylated on tyrosine residues by SRC.
Cytoplasm. Membrane.
Note: Predominantly cytoplasmic. Partially membrane-associated.
Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2 (By similarity). Interacts with CTTN. Interacts (via SH3 domain) with APC.
The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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