产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8794, RRID:AB_2841992.
展开/折叠
2AAA_HUMAN; 6330556D22Rik; Alpha isoform of regulatory subunit A, protein phosphatase; DKFZp470L0914; Medium tumor antigen-associated 61 kDa protein; MGC128399; MGC786; MGC83000; MGC95083; PP2A Aalpha; PP2A; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PP2AAALPHA; Ppp2r1a; ppp2r1a-b; PR65; PR65-alpha; PR65A; Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a; Protein phosphatase 2 (formerly 2A), regulatory subunit A,; Protein phosphatase 2 regulatory subunit A alpha; Protein phosphatase 2, 65-KD regulatory subunit A, alpha; Protein phosphatase 2, regulatory subunit A, alpha isoform; Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform;
抗原和靶标
- P30153 2AAA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30153 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| R134 | Methylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| R167 | Methylation | Uniprot | |
| K188 | Acetylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| C390 | S-Nitrosylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K442 | Sumoylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| Y495 | Phosphorylation | Uniprot | |
| K519 | Ubiquitination | Uniprot | |
| R527 | Methylation | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| K561 | Acetylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| T584 | Phosphorylation | Uniprot |
研究背景
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.
Cytoplasm. Nucleus. Chromosome>Centromere. Lateral cell membrane. Cell projection>Dendrite.
Note: Centromeric localization requires the presence of BUB1.
Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGO1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Interacts with CTTNBP2NL. Interacts with GNA12; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT. Interacts with CIP2A; this interaction stabilizes CIP2A. Interacts with FAM122A. Interacts with ADCY8; antagonizes interaction between ADCY8 and calmodulin (By similarity). Interacts with CRTC3 (when phosphorylated at 'Ser-391').
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Belongs to the phosphatase 2A regulatory subunit A family.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
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