| 产品: | p63 MAPK 抗体 |
| 货号: | DF8796 |
| 描述: | Rabbit polyclonal antibody to p63 MAPK |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 66 kDa; 66kD(Calculated). |
| 蛋白号: | P31152 |
| RRID: | AB_2841994 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8796, RRID:AB_2841994.
展开/折叠
ERK-4; ERK3; Erk3 related; ERK4; Extracellular signal regulated kinase 4; Extracellular signal-regulated kinase 4; MAP kinase 4; MAP kinase isoform p63; MAPK 4; MAPK4; Mitogen activated protein kinase 4; Mitogen-activated protein kinase 4; MK04_HUMAN; p63 MAPK; p63-MAPK; p63MAPK; PRKM4; Protein kinase, mitogen-activated, 4; Protein-tyrosine kinase ERK3-related; RMNK2;
抗原和靶标
- P31152 MK04_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAVDSRACRKVAVKKIALSDARSMKHALREIKIIRRLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGTLAEEHAKLFMYQLLRGLKYIHSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSSRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAFEADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTGAREDEPASLFLEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLDVFISRALKLCTKPEDLPDNKLGDLNGACIPEHPGDLVQTEAFSKERW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31152 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Q8IW41 (MAPKAPK5) , Q13177 (PAK2) | Uniprot |
| S196 | Phosphorylation | P31152 (MAPK4) | Uniprot |
| Y206 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y424 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| T472 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot |
翻译修饰 - P31152 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P31152 (MAPK4) | S196 | Uniprot |
| Q8IW41 (MAPKAPK5) | T182 | Uniprot |
研究背景
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity).
Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following interaction with MAPKAPK5.
High expression in heart and brain.
Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity).
The FRIEDE motif is required for docking MAPKAPK5.
In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究领域
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
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