产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8831, RRID:AB_2842028.
展开/折叠
Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein X; hnRNP E1; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX; nucleic acid binding protein sub 2.3; Nucleic acid- binding protein SUB2.3; Nucleic acid-binding protein SUB2.3; PCBP1; PCBP1_HUMAN; poly(rC) binding protein 1; Poly(rC)-binding protein 1; rCbinding protein 1;
抗原和靶标
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
- Q15365 PCBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15365 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Sumoylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K31 | Sumoylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K37 | Acetylation | Uniprot | |
| S43 | Phosphorylation | P31751 (AKT2) , P31749 (AKT1) | Uniprot |
| S50 | Phosphorylation | Uniprot | |
| C54 | S-Nitrosylation | Uniprot | |
| T60 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| T99 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| C109 | S-Nitrosylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K115 | Sumoylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T127 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| S154 | Phosphorylation | Uniprot | |
| C163 | S-Nitrosylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| C194 | S-Nitrosylation | Uniprot | |
| C201 | S-Nitrosylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| K268 | Sumoylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| R306 | Methylation | Uniprot | |
| K314 | Sumoylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T330 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| K351 | Acetylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| C355 | S-Nitrosylation | Uniprot | |
| S356 | Phosphorylation | Uniprot |
研究背景
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.
Phosphorylated; lowers poly(rC)-binding activity.
Nucleus. Cytoplasm.
Note: Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
研究领域
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Genetic Information Processing > Transcription > Spliceosome.
限制条款
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