产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8847, RRID:AB_2842044.
展开/折叠
Hipk 1; Hipk1; HIPK1_HUMAN; Homeodomain interacting protein kinase 1; Homeodomain interacting protein kinase 1 like protein; Homeodomain-interacting protein kinase 1; Myak; Nbak 2; Nbak2; Nuclear body associated kinase 2; Nuclear body associated kinase 2b;
抗原和靶标
Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells (PubMed:28289210).
- Q86Z02 HIPK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQANSSHQVANFNIPAYDQGLLLPAPAVEHIVVTAADSSGSAATSTFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVDSNGSVQIIEEHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMTHLLDFPHSNHVKSCFQNMEICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSTWQQLPGVALHNSVQPTAMIPEAMGSGQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLDVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPSSSGLKPRSNVISYVTVNDSPDSDSSLSSPYSTDTLSALRGNSGSVLEGPGRVVADGTGTRTIIVPPLKTQLGDCTVATQASGLLSNKTKPVASVSGQSSGCCITPTGYRAQRGGTSAAQPLNLSQNQQSSAAPTSQERSSNPAPRRQQAFVAPLSQAPYTFQHGSPLHSTGHPHLAPAPAHLPSQAHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86Z02 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| K25 | Sumoylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| K120 | Sumoylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| K855 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| T876 | Phosphorylation | Uniprot | |
| S879 | Phosphorylation | Uniprot | |
| K921 | Ubiquitination | Uniprot | |
| K928 | Ubiquitination | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S967 | Phosphorylation | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| T1027 | Phosphorylation | Uniprot | |
| R1035 | Methylation | Uniprot | |
| S1063 | Phosphorylation | Uniprot | |
| Y1197 | Phosphorylation | Uniprot | |
| S1200 | Phosphorylation | Uniprot | |
| T1202 | Phosphorylation | Uniprot |
研究背景
Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN.
Autophosphorylated. Phosphorylated and activated by JNK1.
Degraded by PARK7 at the protein level.
Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm.
Nucleus. Cytoplasm. Nucleus speckle.
Note: Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation.
Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells.
Interacts with Nkx1-2, Nkx2-5, MYB, PARK7, DAXX and p53/TP53. Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex formation promotes MAP3K5-JNK activation and subsequent apoptosis.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
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