产品: | WBP7 抗体 |
货号: | DF8886 |
描述: | Rabbit polyclonal antibody to WBP7 |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Bovine, Horse, Sheep, Rabbit, Dog |
分子量: | 294kDa; 294kD(Calculated). |
蛋白号: | Q9UMN6 |
RRID: | AB_2842082 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8886, RRID:AB_2842082.
展开/折叠
Histone-lysine N-methyltransferase 2B; HRX2; KIAA0304 gene product; KMT2B; KMT2B_HUMAN; Likely ortholog of mouse WW domain binding protein 7; Lysine N-methyltransferase 2B; mixed lineage leukemia 4; Mixed lineage leukemia gene homolog 2 protein; MLL2; myeloid lymphoid leukemia 4; Myeloid lymphoid or mixed lineage leukemia protein 4; Myeloid/lymphoid or mixed-lineage leukemia protein 4; Trithorax homolog 2; trithorax homologue 2; TRX2; WBP-7; WBP7; WW domain-binding protein 7;
抗原和靶标
Widely expressed. Highest levels in testis. Also found in brain with higher expression in the cerebellum than in any other region, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
- Q9UMN6 KMT2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAGGGSCPGPGSARGRFPGRPRGAGGGGGRGGRGNGAERVRVALRRGGGATGPGGAEPGEDTALLRLLGLRRGLRRLRRLWAGPRVQRGRGRGRGRGWGPSRGCVPEEESSDGESDEEEFQGFHSDEDVAPSSLRSALRSQRGRAPRGRGRKHKTTPLPPPRLADVAPTPPKTPARKRGEEGTERMVQALTELLRRAQAPQAPRSRACEPSTPRRSRGRPPGRPAGPCRRKQQAVVVAEAAVTIPKPEPPPPVVPVKHQTGSWKCKEGPGPGPGTPRRGGQSSRGGRGGRGRGRGGGLPFVIKFVSRAKKVKMGQLSLGLESGQGQGQHEESWQDVPQRRVGSGQGGSPCWKKQEQKLDDEEEEKKEEEEKDKEGEEKEERAVAEEMMPAAEKEEAKLPPPPLTPPAPSPPPPLPPPSTSPPPPLCPPPPPPVSPPPLPSPPPPPAQEEQEESPPPVVPATCSRKRGRPPLTPSQRAEREAARAGPEGTSPPTPTPSTATGGPPEDSPTVAPKSTTFLKNIRQFIMPVVSARSSRVIKTPRRFMDEDPPKPPKVEVSPVLRPPITTSPPVPQEPAPVPSPPRAPTPPSTPVPLPEKRRSILREPTFRWTSLTRELPPPPPAPPPPPAPSPPPAPATSSRRPLLLRAPQFTPSEAHLKIYESVLTPPPLGAPEAPEPEPPPADDSPAEPEPRAVGRTNHLSLPRFAPVVTTPVKAEVSPHGAPALSNGPQTQAQLLQPLQALQTQLLPQALPPPQPQLQPPPSPQQMPPLEKARIAGVGSLPLSGVEEKMFSLLKRAKVQLFKIDQQQQQKVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESPVQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDLATEDTSSASETESVPSRSRRGKVEAAGPGGESEPTGSGGTLAHTPRRSLPSHHGKKMRMARCGHCRGCLRVQDCGSCVNCLDKPKFGGPNTKKQCCVYRKCDKIEARKMERLAKKGRTIVKTLLPWDSDESPEASPGPPGPRRGAGAGGPREEVVAHPGPEEQDSLLQRKSARRCVKQRPSYDIFEDSDDSEPGGPPAPRRRTPRENELPLPEPEEQSRPRKPTLQPVLQLKARRRLDKDALAPGPFASFPNGWTGKQKSPDGVHRVRVDFKEDCDLENVWLMGGLSVLTSVPGGPPMVCLLCASKGLHELVFCQVCCDPFHPFCLEEAERPLPQHHDTWCCRRCKFCHVCGRKGRGSKHLLECERCRHAYHPACLGPSYPTRATRKRRHWICSACVRCKSCGATPGKNWDVEWSGDYSLCPRCTQLYEKGNYCPICTRCYEDNDYESKMMQCAQCDHWVHAKCEGLSDEDYEILSGLPDSVLYTCGPCAGAAQPRWREALSGALQGGLRQVLQGLLSSKVVGPLLLCTQCGPDGKQLHPGPCGLQAVSQRFEDGHYKSVHSFMEDMVGILMRHSEEGETPDRRAGGQMKGLLLKLLESAFGWFDAHDPKYWRRSTRLPNGVLPNAVLPPSLDHVYAQWRQQEPETPESGQPPGDPSAAFQGKDPAAFSHLEDPRQCALCLKYGDADSKEAGRLLYIGQNEWTHVNCAIWSAEVFEENDGSLKNVHAAVARGRQMRCELCLKPGATVGCCLSSCLSNFHFMCARASYCIFQDDKKVFCQKHTDLLDGKEIVNPDGFDVLRRVYVDFEGINFKRKFLTGLEPDAINVLIGSIRIDSLGTLSDLSDCEGRLFPIGYQCSRLYWSTVDARRRCWYRCRILEYRPWGPREEPAHLEAAEENQTIVHSPAPSSEPPGGEDPPLDTDVLVPGAPERHSPIQNLDPPLRPDSGSAPPPAPRSFSGARIKVPNYSPSRRPLGGVSFGPLPSPGSPSSLTHHIPTVGDPDFPAPPRRSRRPSPLAPRPPPSRWASPPLKTSPQLRVPPPTSVVTALTPTSGELAPPGPAPSPPPPEDLGPDFEDMEVVSGLSAADLDFAASLLGTEPFQEEIVAAGAMGSSHGGPGDSSEEESSPTSRYIHFPVTVVSAPGLAPSATPGAPRIEQLDGVDDGTDSEAEAVQQPRGQGTPPSGPGVVRAGVLGAAGDRARPPEDLPSEIVDFVLKNLGGPGDGGAGPREESLPPAPPLANGSQPSQGLTASPADPTRTFAWLPGAPGVRVLSLGPAPEPPKPATSKIILVNKLGQVFVKMAGEGEPVPPPVKQPPLPPTISPTAPTSWTLPPGPLLGVLPVVGVVRPAPPPPPPPLTLVLSSGPASPPRQAIRVKRVSTFSGRSPPAPPPYKAPRLDEDGEASEDTPQVPGLGSGGFSRVRMKTPTVRGVLDLDRPGEPAGEESPGPLQERSPLLPLPEDGPPQVPDGPPDLLLESQWHHYSGEASSSEEEPPSPDDKENQAPKRTGPHLRFEISSEDGFSVEAESLEGAWRTLIEKVQEARGHARLRHLSFSGMSGARLLGIHHDAVIFLAEQLPGAQRCQHYKFRYHQQGEGQEEPPLNPHGAARAEVYLRKCTFDMFNFLASQHRVLPEGATCDEEEDEVQLRSTRRATSLELPMAMRFRHLKKTSKEAVGVYRSAIHGRGLFCKRNIDAGEMVIEYSGIVIRSVLTDKREKFYDGKGIGCYMFRMDDFDVVDATMHGNAARFINHSCEPNCFSRVIHVEGQKHIVIFALRRILRGEELTYDYKFPIEDASNKLPCNCGAKRCRRFLN
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UMN6 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
R17 | Methylation | Uniprot | |
R49 | Methylation | Uniprot | |
T159 | Phosphorylation | Uniprot | |
T172 | Phosphorylation | Uniprot | |
T176 | Phosphorylation | Uniprot | |
K180 | Methylation | Uniprot | |
R181 | Methylation | Uniprot | |
R188 | Methylation | Uniprot | |
S214 | Phosphorylation | Uniprot | |
S219 | Phosphorylation | Uniprot | |
R222 | Methylation | Uniprot | |
T278 | Phosphorylation | Uniprot | |
R280 | Methylation | Uniprot | |
S286 | Phosphorylation | Uniprot | |
S346 | Phosphorylation | Uniprot | |
S351 | Phosphorylation | Uniprot | |
T475 | Phosphorylation | Uniprot | |
T496 | Phosphorylation | Uniprot | |
S500 | Phosphorylation | Uniprot | |
T501 | Phosphorylation | Uniprot | |
S510 | Phosphorylation | Uniprot | |
K541 | Methylation | Uniprot | |
T542 | Phosphorylation | Uniprot | |
S582 | Phosphorylation | Uniprot | |
T588 | Phosphorylation | Uniprot | |
S602 | Phosphorylation | Uniprot | |
S632 | Phosphorylation | Uniprot | |
T653 | Phosphorylation | Uniprot | |
S703 | Phosphorylation | Uniprot | |
T713 | Phosphorylation | Uniprot | |
S786 | Phosphorylation | Uniprot | |
K797 | Methylation | Uniprot | |
K797 | Ubiquitination | Uniprot | |
S821 | Phosphorylation | Uniprot | |
S844 | Phosphorylation | Uniprot | |
S857 | Phosphorylation | Uniprot | |
S861 | Phosphorylation | Uniprot | |
T906 | Phosphorylation | Uniprot | |
S910 | Phosphorylation | Uniprot | |
S913 | Phosphorylation | Uniprot | |
K926 | Acetylation | Uniprot | |
S936 | Phosphorylation | Uniprot | |
S941 | Phosphorylation | Uniprot | |
T948 | Phosphorylation | Uniprot | |
S1032 | Phosphorylation | Uniprot | |
S1035 | Phosphorylation | Uniprot | |
S1039 | Phosphorylation | Uniprot | |
S1069 | Phosphorylation | Uniprot | |
S1085 | Phosphorylation | Uniprot | |
S1092 | Phosphorylation | Uniprot | |
S1095 | Phosphorylation | Uniprot | |
S1122 | Phosphorylation | Uniprot | |
T1128 | Phosphorylation | Uniprot | |
K1161 | Methylation | Uniprot | |
S1164 | Phosphorylation | Uniprot | |
Y1275 | Phosphorylation | Uniprot | |
Y1284 | Phosphorylation | Uniprot | |
S1519 | Phosphorylation | Uniprot | |
T1550 | Phosphorylation | Uniprot | |
K1567 | Acetylation | Uniprot | |
K1692 | Ubiquitination | Uniprot | |
Y1707 | Phosphorylation | Uniprot | |
S1836 | Phosphorylation | Uniprot | |
K1866 | Methylation | Uniprot | |
S1917 | Phosphorylation | Uniprot | |
S1930 | Phosphorylation | Uniprot | |
K1934 | Methylation | Uniprot | |
T1935 | Phosphorylation | Uniprot | |
S1936 | Phosphorylation | Uniprot | |
T2068 | Phosphorylation | Uniprot | |
S2070 | Phosphorylation | Uniprot | |
T2083 | Phosphorylation | Uniprot | |
S2086 | Phosphorylation | Uniprot | |
K2185 | Ubiquitination | Uniprot | |
K2196 | Ubiquitination | Uniprot | |
K2203 | Methylation | Uniprot | |
S2270 | Phosphorylation | Uniprot | |
T2283 | Phosphorylation | Uniprot | |
S2288 | Phosphorylation | Uniprot | |
Y2295 | Phosphorylation | Uniprot | |
K2296 | Acetylation | Uniprot | |
S2318 | Phosphorylation | Uniprot | |
S2348 | Phosphorylation | Uniprot | |
S2455 | Phosphorylation | Uniprot | |
S2573 | Phosphorylation | Uniprot | |
Y2604 | Phosphorylation | Uniprot | |
S2605 | Phosphorylation | Uniprot | |
K2691 | Ubiquitination | Uniprot | |
K2700 | Ubiquitination | Uniprot |
研究背景
Histone methyltransferase that methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).
Nucleus.
Widely expressed. Highest levels in testis. Also found in brain with higher expression in the cerebellum than in any other region, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
Component of the menin-associated histone methyltransferase complex, at least composed of KMT2B/MLL4, ASH2L, RBBP5, WDR5, DPY30, MEN1; the complex interacts with POLR2A and POLR2B via MEN1. Interacts with NFE2. Interacts with KDM6B. Interacts (via WIN motif) with WDR5. Interacts (via MBM motif) with MEN1.
The CXXC zinc finger mediates binding to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
文献引用
Application: WB Species: Human Sample: KATO III cells
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