| 产品: | TRIM33 抗体 |
| 货号: | DF8887 |
| 描述: | Rabbit polyclonal antibody to TRIM33 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 123 kDa; 123kD(Calculated). |
| 蛋白号: | Q9UPN9 |
| RRID: | AB_2842083 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8887, RRID:AB_2842083.
展开/折叠
8030451N04Rik; AI413936; cb1085; DKFZp586K1123; E3 ubiquitin-protein ligase TRIM33; EC 6.3.2.-; Ectodermin; Ectodermin homolog; FLJ11429; FLJ32925; id:ibd2175; MGC136680; mKIAA1113; OTTHUMP00000013662; OTTHUMP00000013663; Protein Rfg7; PTC7; Ret fused gene 7; RET-fused gene 7 protein; RFG7; Rfg7 protein; TF1G; TIF1-gamma; TIF1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; Transcriptional intermediary factor 1 gamma; TRI33_HUMAN; trim33; Tripartite motif containing 33; Tripartite motif containing 33 protein; tripartite motif-containing 33; Tripartite motif-containing protein 33; wu:fc17f10; zgc:136680;
抗原和靶标
Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
- Q9UPN9 TRI33_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAENKGGGEAESGGGGSGSAPVTAGAAGPAAQEAEPPLTAVLVEEEEEEGGRAGAEGGAAGPDDGGVAAASSGSAQAASSPAASVGTGVAGGAVSTPAPAPASAPAPGPSAGPPPGPPASLLDTCAVCQQSLQSRREAEPKLLPCLHSFCLRCLPEPERQLSVPIPGGSNGDIQQVGVIRCPVCRQECRQIDLVDNYFVKDTSEAPSSSDEKSEQVCTSCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESVGASGQRPVFCPVHKQEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKKNYVHFAATQVQNRIKEVNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQMKLLQQQNDITGLSRQVKHVMNFTNWAIASGSSTALLYSKRLITFQLRHILKARCDPVPAANGAIRFHCDPTFWAKNVVNLGNLVIESKPAPGYTPNVVVGQVPPGTNHISKTPGQINLAQLRLQHMQQQVYAQKHQQLQQMRMQQPPAPVPTTTTTTQQHPRQAAPQMLQQQPPRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSGPQYSMMQPHLQRQHSNPGHAGPFPVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQLEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSRGSCGSSGRTAEKTSLSFKSDQVKVKQEPGTEDEICSFSGGVKQEKTEDGRRSACMLSSPESSLTPPLSTNLHLESELDALASLENHVKIEPADMNESCKQSGLSSLVNGKSPIRSLMHRSARIGGDGNNKDDDPNEDWCAVCQNGGDLLCCEKCPKVFHLTCHVPTLLSFPSGDWICTFCRDIGKPEVEYDCDNLQHSKKGKTAQGLSPVDQRKCERLLLYLYCHELSIEFQEPVPASIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRLIFKNCERFNEMMKVVQVYADTQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAPLPEFEQEEDDGEVTEDSDEDFIQPRRKRLKSDERPVHIK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UPN9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S169 | Phosphorylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| K252 | Acetylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K334 | Sumoylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| R440 | Methylation | Uniprot | |
| R458 | Methylation | Uniprot | |
| K481 | Sumoylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| R515 | Methylation | Uniprot | |
| Y524 | Phosphorylation | Uniprot | |
| R535 | Methylation | Uniprot | |
| R555 | Methylation | Uniprot | |
| R577 | Methylation | Uniprot | |
| C582 | S-Nitrosylation | Uniprot | |
| R591 | Methylation | Uniprot | |
| R598 | Methylation | Uniprot | |
| R604 | Methylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| T643 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| K763 | Acetylation | Uniprot | |
| K763 | Sumoylation | Uniprot | |
| K763 | Ubiquitination | Uniprot | |
| S767 | Phosphorylation | Uniprot | |
| K769 | Acetylation | Uniprot | |
| K769 | Sumoylation | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| K776 | Acetylation | Uniprot | |
| K776 | Sumoylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| K793 | Acetylation | Uniprot | |
| K793 | Sumoylation | Uniprot | |
| K793 | Ubiquitination | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| T815 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| T820 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| K861 | Acetylation | Uniprot | |
| K861 | Sumoylation | Uniprot | |
| S862 | Phosphorylation | Uniprot | |
| S949 | Phosphorylation | Uniprot | |
| K950 | Acetylation | Uniprot | |
| K951 | Acetylation | Uniprot | |
| K953 | Acetylation | Uniprot | |
| K953 | Sumoylation | Uniprot | |
| K953 | Ubiquitination | Uniprot | |
| S959 | Phosphorylation | Uniprot | |
| K998 | Ubiquitination | Uniprot | |
| K999 | Ubiquitination | Uniprot | |
| K1007 | Sumoylation | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| K1033 | Ubiquitination | Uniprot | |
| Y1048 | Phosphorylation | Uniprot | |
| T1051 | Phosphorylation | Uniprot | |
| K1057 | Sumoylation | Uniprot | |
| K1057 | Ubiquitination | Uniprot | |
| K1067 | Ubiquitination | Uniprot | |
| T1102 | Phosphorylation | Uniprot | |
| S1105 | Phosphorylation | Uniprot | |
| K1118 | Sumoylation | Uniprot | |
| S1119 | Phosphorylation | Uniprot | |
| K1127 | Acetylation | Uniprot |
研究背景
Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
Sumoylated with SUMO1.
Nucleus.
Note: In discrete nuclear dots resembling nuclear bodies.
Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
Homooligomer and heterooligomer with TRIM24 and TRIM28 family members. Interacts with SMAD4 in unstimulated cells. Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta. Interacts with SMAD2 and SMAD3. Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling.
Belongs to the TRIM/RBCC family.
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