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| 产品: | CDK3 抗体 |
| 货号: | DF8911 |
| 描述: | Rabbit polyclonal antibody to CDK3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Sheep, Rabbit |
| 分子量: | 35 kDa; 35kD(Calculated). |
| 蛋白号: | Q00526 |
| RRID: | AB_2842107 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8911, RRID:AB_2842107.
展开/折叠
Cdk 3; Cdk3; CDK3_HUMAN; CDKN3; Cell division kinase 3; Cell division protein kinase 3; Cyclin dependent kinase 3; Cyclin-dependent kinase 3; OTTHUMP00000206828; p36;
抗原和靶标
- Q00526 CDK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q00526 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| T14 | Phosphorylation | Q00526 (CDK3) | Uniprot |
| Y15 | Phosphorylation | Q00526 (CDK3) | Uniprot |
| Y19 | Phosphorylation | Q00526 (CDK3) | Uniprot |
| K33 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| T160 | Phosphorylation | Q00526 (CDK3) | Uniprot |
| T198 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Q00526 (CDK3) | Uniprot |
| Y236 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot |
翻译修饰 - Q00526 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P03372 (ESR1) | S104 | Uniprot |
| P03372 (ESR1) | S106 | Uniprot |
| P03372 (ESR1) | S118 | Uniprot |
| P05412 (JUN) | S63 | Uniprot |
| P05412 (JUN) | S73 | Uniprot |
| P06400 (RB1) | S807 | Uniprot |
| P06400 (RB1) | S811 | Uniprot |
| P18846 (ATF1) | S63 | Uniprot |
| P43629-1 (KIR3DL1) | S296 | Uniprot |
| Q00526 (CDK3) | T14 | Uniprot |
| Q00526 (CDK3) | Y15 | Uniprot |
| Q00526 (CDK3) | Y19 | Uniprot |
| Q00526 (CDK3) | T160 | Uniprot |
| Q00526 (CDK3) | S207 | Uniprot |
| Q12800 (TFCP2) | S309 | Uniprot |
| Q5TKA1 (LIN9) | T96 | Uniprot |
| Q8TDN4-2 (CABLES1) | S48 | Uniprot |
| Q8TDN4 (CABLES1) | S313 | Uniprot |
研究背景
Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.
Expressed in cancer cell lines and glioblastoma tissue.
Interacts with CABLES1 and CABLES2 (By similarity). Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
文献引用
Application: WB Species: mouse Sample: tumor
Application: WB Species: human Sample: H1299 cells
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