| 产品: | CISH 抗体 |
| 货号: | DF8914 |
| 描述: | Rabbit polyclonal antibody to CISH |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Sheep, Rabbit, Dog |
| 分子量: | 29kDa; 29kD(Calculated). |
| 蛋白号: | Q9NSE2 |
| RRID: | AB_2842110 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8914, RRID:AB_2842110.
展开/折叠
BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling;
抗原和靶标
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
- Q9NSE2 CISH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NSE2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S142 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot |
研究背景
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR).
研究领域
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
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