FGF21 Antibody - #DF8947

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产品: FGF21 抗体
货号: DF8947
描述: Rabbit polyclonal antibody to FGF21
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 22 kDa, 16 kDa; 22kD(Calculated).
蛋白号: Q9NSA1
RRID: AB_2842143

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(92%), Bovine(92%), Horse(100%), Rabbit(80%), Dog(92%)
克隆:
Polyclonal
特异性:
FGF21 Antibody detects endogenous levels of total FGF21.
RRID:
AB_2842143
引用格式: Affinity Biosciences Cat# DF8947, RRID:AB_2842143.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FGF 21; FGF-21; Fgf21; FGF21_HUMAN; FGFL; Fibroblast growth factor 21; PRO10196; UNQ3115;

抗原和靶标

免疫原:
Uniprot:

Q9NSA1(FGF21_HUMAN) >>Visit HPA database.

基因/基因ID:
FGF21(26291)
序列:
MDSDETGFEHSGLWVSVLAGLLLGACQAHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Pig
92
Bovine
92
Dog
92
Rabbit
80
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts (via C-terminus) with KLB; this interaction is direct. Interacts with FGFR4.

蛋白家族:

Belongs to the heparin-binding growth factors family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022 [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 6. Effects ofL. plantarum Q16 on key proteins involved in hepatic energy metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.
2). Lycopene attenuates oxidative stress-induced hepatic dysfunction of insulin signal transduction: involvement of FGF21 and mitochondria. The Journal of Nutritional Biochemistry, 2022 (PubMed: 36057413) [IF=5.6]
3). Theabrownin ameliorates liver inflammation, oxidative stress, and fibrosis in MCD diet-fed C57BL/6J mice. Frontiers in Endocrinology, 2023 [IF=5.2]

Application: WB    Species: Mouse    Sample:

FIGURE 7 Theabrownin increases the expression of FGF21 and reduces the phosphorylation of MAPK p38 in MCD diet-fed mice. (A) IHC for FGF21. (B) FGF 21 protein levels from western blot. (C) Quantification of IHC positive area for FGF21. (D) Quantification of FGF 21 protein levels from western blot. (E) p38 protein level and p38 phosphorylation. (F) FGF21 mRNA level. (G) Quantification of p38 phosphorylation (normalized by total p38). AU, arbitrary units. n=5-11 mice per group. The data are mean ± s.e. (error bars). ***p < 0.001, MCS Ctrl vs. MCD Ctrl. †, p
4). Liraglutide regulates lipid metabolism via FGF21-LKB1-AMPK-ACC1 pathway in white adipose tissues and macrophage of type 2 diabetic mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 33640604) [IF=3.1]

Application: WB    Species: mice    Sample: White adipose tissue (WAT)

Fig. 2. Effects of LRG on adipocyte size and the protein expression in WAT of diabetic mice. (A) Adipocyte size of the mice; (B) Quantification of Fig. 2A; (C) Immunohistochemical assay of WAT; (D) Quantification of Fig. 2C; (E) Protein expression in WAT; (F) Quantification of Fig. 3E n ¼ 6 mice/group. #p < 0.05, ##p < 0.01,###p < 0.001 vs CON; *p < 0.05,**p < 0.01, ***p < 0.001 vs DM.

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