| 产品: | HSP74 抗体 |
| 货号: | DF8955 |
| 描述: | Rabbit polyclonal antibody to HSP74 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 94kDa; 94kD(Calculated). |
| 蛋白号: | P34932 |
| RRID: | AB_2842151 |
产品描述
来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
HSP74 Antibody detects endogenous levels of total HSP74.
RRID:
AB_2842151
引用格式: Affinity Biosciences Cat# DF8955, RRID:AB_2842151.
引用格式: Affinity Biosciences Cat# DF8955, RRID:AB_2842151.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
APG 2; Epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70 related protein APG 2; Heat shock 70-related protein APG-2; Heat shock protein, 110 kDa; HEL S 5a; HS24/P52; hsp70; Hsp70 RY; hsp70RY; HSP74_HUMAN; HSPA4; HSPH2; MGC131852; RY;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- P34932 HSP74_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACISFGPKNRSIGAAAKSQVISNAKNTVQGFKRFHGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDCVVSVPCFYTDAERRSVMDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQVSVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLKKLMSANASDLPLSIECFMNDVDVSGTMNRGKFLEMCNDLLARVEPPLRSVLEQTKLKKEDIYAVEIVGGATRIPAVKEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVFSKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIFSVSSASLVEVHKSEENEEPMETDQNAKEEEKMQVDQEEPHVEEQQQQTPAENKAESEEMETSQAGSKDKKMDQPPQAKKAKVKTSTVDLPIENQLLWQIDREMLNLYIENEGKMIMQDKLEKERNDAKNAVEEYVYEMRDKLSGEYEKFVSEDDRNSFTLKLEDTENWLYEDGEDQPKQVYVDKLAELKNLGQPIKIRFQESEERPKLFEELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKVEPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMDID
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P34932 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| R33 | Methylation | Uniprot | |
| C34 | S-Nitrosylation | Uniprot | |
| C38 | S-Nitrosylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| T113 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| Y184 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K194 | Acetylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| C245 | S-Nitrosylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| R265 | Methylation | Uniprot | |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K329 | Acetylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K356 | Acetylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| K388 | Methylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| C417 | S-Nitrosylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| K430 | Acetylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| T433 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| K437 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| T538 | Phosphorylation | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| K557 | Methylation | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K559 | Methylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K573 | Ubiquitination | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| K603 | Acetylation | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| Y624 | Phosphorylation | Uniprot | |
| Y626 | Phosphorylation | Uniprot | |
| K631 | Acetylation | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| Y636 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| R645 | Methylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| Y660 | Phosphorylation | Uniprot | |
| K668 | Ubiquitination | Uniprot | |
| Y671 | Phosphorylation | Uniprot | |
| K674 | Acetylation | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| K679 | Acetylation | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K686 | Ubiquitination | Uniprot | |
| S692 | Phosphorylation | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| K704 | Ubiquitination | Uniprot | |
| K711 | Ubiquitination | Uniprot | |
| K717 | Ubiquitination | Uniprot | |
| K719 | Ubiquitination | Uniprot | |
| Y723 | Phosphorylation | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| S756 | Phosphorylation | Uniprot | |
| K773 | Methylation | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| K794 | Ubiquitination | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| S830 | Phosphorylation | Uniprot |
研究背景
细胞定位:
Cytoplasm.
亚基结构:
Interacts with TJP1/ZO-1.
蛋白家族:
Belongs to the heat shock protein 70 family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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