INSIG2 Antibody - #DF8990

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产品: INSIG2 抗体
货号: DF8990
描述: Rabbit polyclonal antibody to INSIG2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 25 kDa; 25kD(Calculated).
蛋白号: Q9Y5U4
RRID: AB_2842186

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(100%), Dog(90%), Chicken(80%)
克隆:
Polyclonal
特异性:
INSIG2 Antibody detects endogenous levels of total INSIG2.
RRID:
AB_2842186
引用格式: Affinity Biosciences Cat# DF8990, RRID:AB_2842186.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Hypothetical protein INSIG2; INSIG 2; INSIG 2 membrane protein; INSIG2 membrane protein; Insulin induced gene 2; Insulin induced gene 2 protein; Insulin induced protein 2; MGC26273;

抗原和靶标

免疫原:
Uniprot:

Q9Y5U4(INSI2_HUMAN) >>Visit HPA database.

基因/基因ID:
INSIG2(51141)
序列:
MAEGETESPGPKKCGPYISSVTSQSVNLMIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVIASIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNIQLSLTLAALSIGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
100
Horse
90
Bovine
90
Sheep
90
Dog
90
Chicken
80
Zebrafish
60
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y5U4 作为底物

Site PTM Type Enzyme
K100 Ubiquitination
K102 Ubiquitination
K221 Ubiquitination

研究背景

功能:

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR.

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with RNF145.

蛋白家族:

Belongs to the INSIG family.

文献引用

1). Ginsenosides From Panax ginseng Improves Hepatic Lipid Metabolism Disorders in HFD-Fed Rats by Regulating Gut Microbiota and Cholesterol Metabolism Signaling Pathways. Phytotherapy research : PTR, 2024 (PubMed: 39660634) [IF=6.1]

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