产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9013, RRID:AB_2842209.
展开/折叠
Hair keratin K2.10; Hard keratin, type II, 3; HB 3; HB3; hHb3; K2.10; K83; Keratin 83; Keratin; Keratin, hair, basic, 3; Keratin-83; Krt83; KRT83_HUMAN; KRTHB3; type II cuticular Hb3; Type II hair keratin Hb3; Type-II keratin Kb23; 1; basic; ghHb 1; ghHb1; ghHkb 1; ghHKb1; hair; Hair keratin K2.9; Hard keratin type II 1; HB 1; HB1; hHAKB2 1; K2.9; K81; Keratin 81; Keratin; Keratin hair basic 1; Keratin type II cuticular Hb1; Keratin-81; Keratin81; KRT 81; KRT81; KRT81_HUMAN; KRTHB 1; KRTHB1; Metastatic lymph node 137 gene protein; MLN 137; MLN137; type II cuticular Hb1; Type II hair keratin Hb1; Type-II keratin Kb21; ECTD4; Hair keratin K2.12; Hard keratin type II 5; Hb 5; HB5; hHb5; K12.12; K85; Keratin 85; keratin hair basic 5; Keratin type II cuticular Hb5; keratin, hard, type II; KRTHB5; type II cuticular Hb5; Type II hair keratin Hb5; Type II keratin Kb25; Hair keratin K2.11; Hb6; K86; Keratin; Keratin-86; KRT86; KRT86_HUMAN; KRTHB6; MNX; type II cuticular Hb6; Type II hair keratin Hb6; Type-II keratin Kb26;
抗原和靶标
P78385(KRT83_HUMAN) >>Visit HPA database.
Q14533(KRT81_HUMAN) >>Visit HPA database.
Synthesis begins in the cortex 10-15 cell layers above the apex of the dermal papilla and ends abruptly in the middle of the cortex.
Q14533 KRT81_HUMAN:Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue.
P78386 KRT85_HUMAN:Synthesis occurs immediately above a small population of matrix cells at the base of the hair bulb and the trichocytes lining the dermal papilla and extends upward through the matrix and ends in the lower part of the cortex of the hair shaft.
O43790 KRT86_HUMAN:Synthesis begins slightly higher in the hair shaft than HB1 and HB3 and continues much farther up, ending in the keratogeneous zone.
- P78385 KRT83_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTCGFNSIGCGFRPGNFSCVSACGPRPSRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFAGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRILQSHISDTSVVVKLDNSRDLNMDCIVAEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVEAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNLVVSTGLCKPCGQLNTTCGGGSCGQGRH
- Q14533 KRT81_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTCGSGFGGRAFSCISACGPRPGRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFEGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVSLRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEILILQSHISDTSVVVKLDNSRDLNMDCIIAEIKAQYDDIVTRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGIGAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNVAVSTGLCAPCGQLNTTCGGGSCGVGSCGISSLGVGSCGSSCRKC
- P78386 KRT85_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSCRSYRISSGCGVTRNFSSCSAVAPKTGNRCCISAAPYRGVSCYRGLTGFGSRSLCNLGSCGPRIAVGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKWQFYQNQRCCESNLEPLFSGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVVLKKDVDCAYLRKSDLEANVEALVEESSFLRRLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDCIIAEIKAQYDDVASRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEIENAKCQRAKLEAAVAEAEQQGEAALSDARCKLAELEGALQKAKQDMACLLKEYQEVMNSKLGLDIEIATYRRLLEGEEHRLCEGVGSVNVCVSSSRGGVSCGGLSYSTTPGRQITSGPSAIGGSITVVAPDSCAPCQPRSSSFSCGSSRSVRFA
- O43790 KRT86_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTCGSYCGGRAFSCISACGPRPGRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFEGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVSLRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRVLQSHISDTSVVVKLDNSRDLNMDCIIAEIKAQYDDIVTRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVGSVNVCVSSSRGGVVCGDLCASTTAPVVSTRVSSVPSNSNVVVGTTNACAPSARVGVCGGSCKRC
翻译修饰 - P78385/Q14533/P78386/O43790 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Phosphorylation | Uniprot | |
K149 | Ubiquitination | Uniprot | |
K229 | Sumoylation | Uniprot | |
K229 | Ubiquitination | Uniprot | |
K230 | Ubiquitination | Uniprot | |
Y260 | Phosphorylation | Uniprot | |
Y314 | Phosphorylation | Uniprot | |
K317 | Ubiquitination | Uniprot | |
K322 | Ubiquitination | Uniprot | |
T324 | Phosphorylation | Uniprot | |
T331 | Phosphorylation | Uniprot | |
T335 | Phosphorylation | Uniprot | |
K336 | Ubiquitination | Uniprot | |
K384 | Ubiquitination | Uniprot | |
K394 | Ubiquitination | Uniprot | |
K396 | Ubiquitination | Uniprot | |
S412 | Phosphorylation | Uniprot | |
S493 | Phosphorylation | Uniprot | |
S494 | Phosphorylation | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S47 | Phosphorylation | Uniprot | |
S49 | Phosphorylation | Uniprot | |
K132 | Ubiquitination | Uniprot | |
K193 | Methylation | Uniprot | |
T203 | Phosphorylation | Uniprot | |
K222 | Methylation | Uniprot | |
Y243 | Phosphorylation | Uniprot | |
Y282 | Phosphorylation | Uniprot | |
Y297 | Phosphorylation | Uniprot | |
K300 | Ubiquitination | Uniprot | |
K305 | Ubiquitination | Uniprot | |
T307 | Phosphorylation | Uniprot | |
T314 | Phosphorylation | Uniprot | |
T318 | Phosphorylation | Uniprot | |
K319 | Ubiquitination | Uniprot | |
K340 | Ubiquitination | Uniprot | |
K345 | Ubiquitination | Uniprot | |
K367 | Ubiquitination | Uniprot | |
K377 | Methylation | Uniprot | |
K377 | Ubiquitination | Uniprot | |
K379 | Ubiquitination | Uniprot | |
S395 | Phosphorylation | Uniprot | |
S455 | Phosphorylation | Uniprot | |
S460 | Phosphorylation | Uniprot | |
T466 | Phosphorylation | Uniprot | |
S482 | Phosphorylation | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S52 | Phosphorylation | Uniprot | |
S54 | Phosphorylation | Uniprot | |
K137 | Ubiquitination | Uniprot | |
Y248 | Phosphorylation | Uniprot | |
S257 | Phosphorylation | Uniprot | |
S263 | Phosphorylation | Uniprot | |
Y302 | Phosphorylation | Uniprot | |
T312 | Phosphorylation | Uniprot | |
T319 | Phosphorylation | Uniprot | |
T323 | Phosphorylation | Uniprot | |
K372 | Ubiquitination | Uniprot | |
S400 | Phosphorylation | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S47 | Phosphorylation | Uniprot | |
S49 | Phosphorylation | Uniprot | |
K132 | Ubiquitination | Uniprot | |
T203 | Phosphorylation | Uniprot | |
S252 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
Y282 | Phosphorylation | Uniprot | |
Y297 | Phosphorylation | Uniprot | |
K300 | Ubiquitination | Uniprot | |
K305 | Ubiquitination | Uniprot | |
T307 | Phosphorylation | Uniprot | |
T314 | Phosphorylation | Uniprot | |
T318 | Phosphorylation | Uniprot | |
K319 | Ubiquitination | Uniprot | |
K340 | Ubiquitination | Uniprot | |
K345 | Ubiquitination | Uniprot | |
K367 | Ubiquitination | Uniprot | |
K377 | Ubiquitination | Uniprot | |
K379 | Ubiquitination | Uniprot | |
S395 | Phosphorylation | Uniprot |
研究背景
Synthesis begins in the cortex 10-15 cell layers above the apex of the dermal papilla and ends abruptly in the middle of the cortex.
Heterotetramer of two type I and two type II keratins.
Belongs to the intermediate filament family.
Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue.
Heterotetramer of two type I and two type II keratins.
Belongs to the intermediate filament family.
Synthesis occurs immediately above a small population of matrix cells at the base of the hair bulb and the trichocytes lining the dermal papilla and extends upward through the matrix and ends in the lower part of the cortex of the hair shaft.
Heterotetramer of two type I and two type II keratins.
Belongs to the intermediate filament family.
Synthesis begins slightly higher in the hair shaft than HB1 and HB3 and continues much farther up, ending in the keratogeneous zone.
Heterotetramer of two type I and two type II keratins.
Belongs to the intermediate filament family.
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