MYLK2 Antibody - #DF9023

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产品: MYLK2 抗体
货号: DF9023
描述: Rabbit polyclonal antibody to MYLK2
应用: WB IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 65 kDa; 65kD(Calculated).
蛋白号: Q9H1R3
RRID: AB_2842219

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
MYLK2 Antibody detects endogenous levels of total MYLK2.
RRID:
AB_2842219
引用格式: Affinity Biosciences Cat# DF9023, RRID:AB_2842219.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

KMLC; MLCK; MLCK, skeletal/cardiac; MLCK2; MYLK 2; MYLK2; MYLK2_HUMAN; Myosin light chain kinase 2; Myosin light chain kinase 2 skeletal muscle; Myosin light chain kinase 2 skeletal/cardiac muscle; MYOSIN LIGHT POLYPEPTIDE KINASE; Myosin light polypeptide kinase, skeletal/cardiac; Skeletal muscle myosin light chain kinase; Skeletal myosin light chain kinase; skeletal/cardiac muscle; skMLCK;

抗原和靶标

免疫原:
Uniprot:

Q9H1R3(MYLK2_HUMAN) >>Visit HPA database.

基因/基因ID:
MYLK2(85366)
表达:
Q9H1R3 MYLK2_HUMAN:

Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.

序列:
MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVCTCMEKATGLKLAAKVIKKQTPKDKEMVLLEIEVMNQLNHRNLIQLYAAIETPHEIVLFMEYIEGGELFERIVDEDYHLTEVDTMVFVRQICDGILFMHKMRVLHLDLKPENILCVNTTGHLVKIIDFGLARRYNPNEKLKVNFGTPEFLSPEVVNYDQISDKTDMWSMGVITYMLLSGLSPFLGDDDTETLNNVLSGNWYFDEETFEAVSDEAKDFVSNLIVKDQRARMNAAQCLAHPWLNNLAEKAKRCNRRLKSQILLKKYLMKRRWKKNFIAVSAANRFKKISSSGALMALGV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9H1R3 作为底物

Site PTM Type Enzyme
S143 Phosphorylation
S151 Phosphorylation P49137 (MAPKAPK2)
K561 Ubiquitination
Y563 Phosphorylation
K584 Ubiquitination
S586 Phosphorylation
S587 Phosphorylation
S588 Phosphorylation

翻译修饰 - Q9H1R3 作为激酶

Substrate Site Source
Q06413 (MEF2C) T80 Uniprot

研究背景

功能:

Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.

细胞定位:

Cytoplasm.
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.

亚基结构:

May interact with centrin.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

文献引用

1). Inhibition of Peripheral ERK Signaling Ameliorates Persistent Muscle Pain Around Trigger Points in Rats. Cell Transplantation, 2020 (PubMed: 33081508) [IF=3.3]

Application: WB    Species: rat    Sample: muscle

Figure 6. |MLCK might be the target of the ERK inhibitor involved in ameliorating muscular mechanical hyperalgesia. (B) The expression of MLCK protein. MLCK expression was increased in the MPS group compared with that in the control group. MLCK expression was decreased in the ERK inhibitor group compared with that in the MPS group.

Application: WB    Species: Rat    Sample: muscle tissue

Figure 6. MLCK might be the target of the ERK inhibitor involved in ameliorating muscular mechanical hyperalgesia. (A) The relationship between ERK and MLCK was shown by bioinformatics prediction with the STRING database. (B) The expression of MLCK protein. MLCK expression was increased in the MPS group compared with that in the control group. MLCK expression was decreased in the ERK inhibitor group compared with that in the MPS group. Data are presented as the mean ± SEM (n = 6). The data were analyzed using one-way ANOVA. **P < 0.01. (C) The expression of p-MLC protein. p-MLC expression was increased in the MPS group compared with that in the control group. p-MLC expression was decreased in the ERK inhibitor group compared with that in the MPS group. Data are presented as the mean ± SEM (n = 6). The data were analyzed using one-way ANOVA. **P < 0.01. ANOVA: analysis of variance; MLCK: myosin light chain kinase; MPS: myofascial pain syndrome; SEM: standard error of the mean.
2). Dexmedetomidine Inhibited Peripheral Platelet-Derived Growth Factor Receptor Α Regulated Myofascial Trigger Points Pain and Muscle Fibers Contraction Via JAK2/STAT3 Signaling Pathway. , 2022

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