| 产品: | TNIK 抗体 |
| 货号: | DF9050 |
| 描述: | Rabbit polyclonal antibody to TNIK |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 155 kDa; 155kD(Calculated). |
| 蛋白号: | Q9UKE5 |
| RRID: | AB_2842246 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9050, RRID:AB_2842246.
展开/折叠
EC 2.7.11.1; KIAA0551; Tnik; TNIK_HUMAN; Traf2 and NCK interacting kinase; Traf2 and NCK-interacting protein kinase;
抗原和靶标
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
- Q9UKE5 TNIK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPILESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPAKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPMKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UKE5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y323 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| Y475 | Phosphorylation | Uniprot | |
| Y499 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| Y519 | Phosphorylation | Uniprot | |
| Y521 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| T643 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| T677 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| R698 | Methylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| R705 | Methylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| K824 | Acetylation | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| T902 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| T942 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| S959 | Phosphorylation | Uniprot | |
| Y963 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| Y982 | Phosphorylation | Uniprot | |
| T987 | Phosphorylation | Uniprot | |
| S1021 | Phosphorylation | Uniprot | |
| T1036 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | Uniprot | |
| K1131 | Ubiquitination | Uniprot | |
| K1178 | Ubiquitination | Uniprot | |
| Y1264 | Phosphorylation | Uniprot | |
| T1267 | Phosphorylation | Uniprot | |
| K1319 | Ubiquitination | Uniprot | |
| K1325 | Ubiquitination | Uniprot | |
| K1333 | Ubiquitination | Uniprot |
翻译修饰 - Q9UKE5 作为激酶
研究背景
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
Nucleus. Cytoplasm. Recycling endosome. Cytoplasm>Cytoskeleton.
Note: Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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