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Western blot analysis of extracts from 293 cells(UV treatment), using ACINU Antibody.

Western blot analysis of extracts from Huvec, using ACINU Antibody.

产品:	ACINU 抗体
货号:	DF9051
描述:	Rabbit polyclonal antibody to ACINU
应用:	WB
反应:	Human, Mouse, Rat, Monkey
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	152 kDa; 152kD(Calculated).
蛋白号:	Q9UKV3
RRID:	AB_2842247

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Monkey

预测:

Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)

克隆:

Polyclonal

特异性:

ACINU Antibody detects endogenous levels of total ACINU.

RRID:

AB_2842247
引用格式: Affinity Biosciences Cat# DF9051, RRID:AB_2842247.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670;

抗原和靶标

免疫原:

Uniprot:

Q9UKV3(ACINU_HUMAN) >>Visit HPA database.

基因/基因ID:

ACIN1(22985)

表达:

Q9UKV3 ACINU_HUMAN:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

序列:

Q9UKV3 ACINU_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGKPLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQYLEKQQELLRQRLEREAREAAELEEASAESEDEMIHPEGVASLLPPDFQSSLERPELELSRHSPRKSSSISEEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEEDQETPSRNLRVRADRNLKTEEEEEEEEEEEEDDEEEEGDDEGQKSREAPILKEFKEEGEEIPRVKPEEMMDERPKTRSQEQEVLERGGRFTRSQEEARKSHLARQQQEKEMKTTSPLEEEEREIKSSQGLKEKSKSPSPPRLTEDRKKASLVALPEQTASEEETPPPLLTKEASSPPPHPQLHSEEEIEPMEGPAPAVLIQLSPPNTDADTRELLVSQHTVQLVGGLSPLSSPSDTKAESPAEKVPEESVLPLVQKSTLADYSAQKDLEPESDRSAQPLPLKIEELALAKGITEECLKQPSLEQKEGRRASHTLLPSHRLKQSADSSSSRSSSSSSSSSRSRSRSPDSSGSRSHSPLRSKQRDVAQARTHANPRGRPKMGSRSTSESRSRSRSRSRSASSNSRKSLSPGVSRDSSTSYTETKDPSSGQEVATPPVPQLQVCEPKERTSTSSSSVQARRLSQPESAEKHVTQRLQPERGSPKKCEAEEAEPPAATQPQTSETQTSHLPESERIHHTVEEKEEVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEEKKESSLPKSFKRKISVVSATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREEEEEEKEPEAEPPVPPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UKV3 作为底物

Q9UKV3

Site	PTM Type	Enzyme	Source
K5	Ubiquitination		Uniprot
R8	Methylation		Uniprot
R14	Methylation		Uniprot
S18	Phosphorylation		Uniprot
R29	Methylation		Uniprot
K81	Methylation		Uniprot
K81	Ubiquitination		Uniprot
R87	Methylation		Uniprot
S96	Phosphorylation		Uniprot
K103	Methylation		Uniprot
K103	Ubiquitination		Uniprot
K113	Methylation		Uniprot
S115	Phosphorylation		Uniprot
T116	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
S132	Phosphorylation		Uniprot
S135	Phosphorylation		Uniprot
K138	Ubiquitination		Uniprot
K143	Methylation		Uniprot
K143	Ubiquitination		Uniprot
S166	Phosphorylation		Uniprot
S169	Phosphorylation		Uniprot
S189	Phosphorylation		Uniprot
S190	Phosphorylation		Uniprot
S199	Phosphorylation		Uniprot
S206	Phosphorylation		Uniprot
S208	Phosphorylation		Uniprot
S210	Phosphorylation		Uniprot
S216	Phosphorylation		Uniprot
S228	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
K238	Acetylation		Uniprot
S240	Phosphorylation		Uniprot
S243	Phosphorylation		Uniprot
T254	Phosphorylation		Uniprot
S256	Phosphorylation		Uniprot
K268	Sumoylation		Uniprot
T269	Phosphorylation		Uniprot
S295	Phosphorylation		Uniprot
K302	Ubiquitination		Uniprot
K315	Sumoylation		Uniprot
T326	Phosphorylation		Uniprot
S328	Phosphorylation		Uniprot
R336	Methylation		Uniprot
T341	Phosphorylation		Uniprot
S343	Phosphorylation		Uniprot
K359	Acetylation		Uniprot
T363	Phosphorylation		Uniprot
T364	Phosphorylation		Uniprot
S365	Phosphorylation		Uniprot
S376	Phosphorylation		Uniprot
S377	Phosphorylation		Uniprot
S384	Phosphorylation		Uniprot
S386	Phosphorylation		Uniprot
S388	Phosphorylation		Uniprot
T393	Phosphorylation		Uniprot
S400	Phosphorylation		Uniprot
T408	Phosphorylation		Uniprot
S410	Phosphorylation		Uniprot
T414	Phosphorylation		Uniprot
T420	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot
S425	Phosphorylation		Uniprot
S434	Phosphorylation		Uniprot
S453	Phosphorylation		Uniprot
T457	Phosphorylation		Uniprot
T461	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot
S481	Phosphorylation		Uniprot
S482	Phosphorylation		Uniprot
S484	Phosphorylation		Uniprot
T486	Phosphorylation		Uniprot
S490	Phosphorylation		Uniprot
S499	Phosphorylation		Uniprot
K506	Ubiquitination		Uniprot
Y512	Phosphorylation		Uniprot
S513	Phosphorylation		Uniprot
K516	Ubiquitination		Uniprot
S522	Phosphorylation		Uniprot
R524	Methylation		Uniprot
S525	Phosphorylation		Uniprot
K532	Sumoylation		Uniprot
K540	Ubiquitination		Uniprot
K548	Ubiquitination		Uniprot
S551	Phosphorylation		Uniprot
S561	Phosphorylation		Uniprot
T563	Phosphorylation		Uniprot
S583	Phosphorylation		Uniprot
S584	Phosphorylation		Uniprot
S585	Phosphorylation		Uniprot
S586	Phosphorylation		Uniprot
S588	Phosphorylation		Uniprot
S595	Phosphorylation		Uniprot
S599	Phosphorylation		Uniprot
S601	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
S635	Phosphorylation		Uniprot
S637	Phosphorylation		Uniprot
S645	Phosphorylation		Uniprot
K654	Methylation		Uniprot
S655	Phosphorylation		Uniprot
S657	Phosphorylation		Uniprot
S661	Phosphorylation		Uniprot
S664	Phosphorylation		Uniprot
T666	Phosphorylation		Uniprot
Y668	Phosphorylation		Uniprot
T671	Phosphorylation		Uniprot
S675	Phosphorylation		Uniprot
S676	Phosphorylation		Uniprot
T682	Phosphorylation		Uniprot
C691	S-Nitrosylation		Uniprot
T697	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot
T699	Phosphorylation		Uniprot
S702	Phosphorylation		Uniprot
S703	Phosphorylation		Uniprot
S710	Phosphorylation		Uniprot
S714	Phosphorylation		Uniprot
K717	Acetylation		Uniprot
T720	Phosphorylation		Uniprot
S729	Phosphorylation		Uniprot
T744	Phosphorylation		Uniprot
T748	Phosphorylation		Uniprot
T751	Phosphorylation		Uniprot
S754	Phosphorylation		Uniprot
S814	Phosphorylation		Uniprot
S815	Phosphorylation		Uniprot
K818	Ubiquitination		Uniprot
S819	Phosphorylation		Uniprot
K823	Ubiquitination		Uniprot
S825	Phosphorylation		Uniprot
S828	Phosphorylation		Uniprot
T830	Phosphorylation		Uniprot
K831	Ubiquitination		Uniprot
S838	Phosphorylation		Uniprot
T840	Phosphorylation		Uniprot
K860	Ubiquitination		Uniprot
K861	Ubiquitination		Uniprot
S863	Phosphorylation		Uniprot
S865	Phosphorylation		Uniprot
S895	Phosphorylation		Uniprot
S898	Phosphorylation		Uniprot
T902	Phosphorylation		Uniprot
T976	Phosphorylation		Uniprot
T978	Phosphorylation		Uniprot
S981	Phosphorylation		Uniprot
S983	Phosphorylation		Uniprot
K986	Ubiquitination		Uniprot
S987	Phosphorylation		Uniprot
S990	Phosphorylation		Uniprot
T992	Phosphorylation		Uniprot
T999	Phosphorylation		Uniprot
S1004	Phosphorylation		Uniprot
K1029	Ubiquitination		Uniprot
K1047	Ubiquitination		Uniprot
S1104	Phosphorylation		Uniprot
T1106	Phosphorylation		Uniprot
S1161	Phosphorylation		Uniprot
S1176	Phosphorylation		Uniprot
S1178	Phosphorylation		Uniprot
S1180	Phosphorylation	P78362 (SRPK2) , P31749 (AKT1)	Uniprot
K1209	Ubiquitination		Uniprot
K1219	Ubiquitination		Uniprot
Y1225	Phosphorylation		Uniprot
T1230	Phosphorylation		Uniprot
S1232	Phosphorylation		Uniprot
K1237	Ubiquitination		Uniprot
K1253	Methylation		Uniprot
S1284	Phosphorylation		Uniprot
S1325	Phosphorylation		Uniprot
S1327	Phosphorylation		Uniprot
S1329	Phosphorylation	P31749 (AKT1)	Uniprot
S1331	Phosphorylation		Uniprot
T1332	Phosphorylation		Uniprot

研究背景

Q9UKV3_ACINU_HUMAN

功能:

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

翻译修饰:

Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1.

Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.

细胞定位:

Nucleus. Nucleus speckle. Nucleus>Nucleoplasm.
Note: Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

组织特异性:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

亚基结构:

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.

研究领域

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

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ACINU Antibody - #DF9051

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