RAD54B Antibody - #DF9055

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产品: RAD54B 抗体
货号: DF9055
描述: Rabbit polyclonal antibody to RAD54B
应用: WB
反应: Human
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
分子量: 102 kDa; 103kD(Calculated).
蛋白号: Q9Y620
RRID: AB_2842251

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(91%), Zebrafish(83%), Bovine(82%), Sheep(91%), Rabbit(91%), Dog(89%)
克隆:
Polyclonal
特异性:
RAD54B Antibody detects endogenous levels of total RAD54B.
RRID:
AB_2842251
引用格式: Affinity Biosciences Cat# DF9055, RRID:AB_2842251.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA repair and recombination protein RAD54B; EC 3.6.1.-; Fibrinogen silencer binding protein; FSBP; RAD54 homolog B;

抗原和靶标

免疫原:
Uniprot:

Q9Y620(RA54B_HUMAN) >>Visit HPA database.

基因/基因ID:
RAD54B(25788)
表达:
Q9Y620 RA54B_HUMAN:

Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon.

序列:
MRRSAAPSQLQGNSFKKPKFIPPGRSNPGLNEEITKLNPDIKLFEGVAINNTFLPSQNDLRICSLNLPSEESTREINNRDNCSGKYCFEAPTLATLDPPHTVHSAPKEVAVSKEQEEKSDSLVKYFSVVWCKPSKKKHKKWEGDAVLIVKGKSFILKNLEGKDIGRGIGYKFKELEKIEEGQTLMICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPRHDPYTPNSLVMPRPDKNHQWVFNKNCFPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAILADEMGLGKTLQCISLIWTLQCQGPYGGKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKVEEFIKSIFYSVLIISYEMLLRSLDQIKNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQNDLQEFFALIDFVNPGILGSLSSYRKIYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEIINKYLPPKIENVVFCRPGALQIELYRKLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKEKECSSTCDKNEEKSLYKGLLSVFPADYNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLNILQEVCKRHGYAYTRLDGQTPISQRQQIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNPATDIQAMSRVWRDGQKYPVHIYRLLTTGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
91
Sheep
91
Rabbit
91
Dog
89
Zebrafish
83
Bovine
82
Chicken
75
Xenopus
70
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y620 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
S14 Phosphorylation
K36 Ubiquitination
K157 Ubiquitination
K162 Ubiquitination
K226 Methylation
K226 Ubiquitination
S229 Phosphorylation
K252 Ubiquitination
K270 Ubiquitination
K371 Ubiquitination
K376 Ubiquitination
K384 Ubiquitination
S409 Phosphorylation
K490 Ubiquitination
Y492 Phosphorylation
K590 Ubiquitination
K603 Ubiquitination
S608 Phosphorylation
S609 Phosphorylation
T610 Phosphorylation
K621 Ubiquitination
K639 Ubiquitination
K643 Acetylation
K643 Ubiquitination
S648 Phosphorylation
Y761 Phosphorylation
K777 Ubiquitination
K785 Ubiquitination
K864 Ubiquitination
K872 Ubiquitination
K875 Ubiquitination

研究背景

功能:

Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon.

亚基结构:

Interacts with RAD51 through the NH2-terminal domain. Immunoprecipitation experiments show that the interaction is constitutive and not induced by ionizing radiation. The interaction may be indirect.

蛋白家族:

Belongs to the SNF2/RAD54 helicase family.

研究领域

· Genetic Information Processing > Replication and repair > Homologous recombination.

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