| 产品: | GEMIN8 抗体 |
| 货号: | DF9077 |
| 描述: | Rabbit polyclonal antibody to GEMIN8 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 29kDa; 29kD(Calculated). |
| 蛋白号: | Q9NWZ8 |
| RRID: | AB_2842273 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9077, RRID:AB_2842273.
展开/折叠
FAM51A1; FLJ20514; FLJ43072; gem (nuclear organelle) associated protein 8;
抗原和靶标
- Q9NWZ8 GEMI8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NWZ8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot |
研究背景
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
Nucleus>Gem. Cytoplasm.
Note: Found in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.
Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1 and with the GEMIN6-GEMIN7 heterodimer, mediating their interaction.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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