产品: RPL27 抗体
货号: DF9129
描述: Rabbit polyclonal antibody to RPL27
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 16 kDa; 16kD(Calculated).
蛋白号: P61353
RRID: AB_2842325

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 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
RPL27 Antibody detects endogenous levels of total RPL27.
RRID:
AB_2842325
引用格式: Affinity Biosciences Cat# DF9129, RRID:AB_2842325.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L27; Ribosomal protein L27; RPL 27;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
92
Zebrafish
92
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P61353 作为底物

Site PTM Type Enzyme
K6 Ubiquitination
K9 Ubiquitination
K27 Acetylation
K27 Ubiquitination
R36 Methylation
Y49 Phosphorylation
K52 Ubiquitination
K59 Ubiquitination
K73 Ubiquitination
Y75 Phosphorylation
Y77 Phosphorylation
T83 Phosphorylation
R84 Methylation
S86 Phosphorylation
K93 Acetylation
K93 Ubiquitination
K98 Acetylation
K98 Sumoylation
K98 Ubiquitination
K107 Ubiquitination
K115 Ubiquitination
K117 Acetylation
K117 Sumoylation
K117 Ubiquitination
K128 Acetylation
K128 Ubiquitination
K133 Acetylation
K133 Ubiquitination

研究背景

功能:

Component of the large ribosomal subunit. Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs.

细胞定位:

Cytoplasm>Cytosol. Cytoplasm. Rough endoplasmic reticulum.
Note: Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the large ribosomal subunit. Interacts with RRP1B. Interacts with DHX33.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eL27 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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