产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9139, RRID:AB_2842335.
展开/折叠
5-bisphosphate 5-phosphatase; 72 kDa inositol polyphosphate 5-phosphatase; Inositol polyphosphate 5 phosphatase; INP5E_HUMAN; Inpp5e; Phosphatidylinositol (4,5) bisphosphate 5 phosphatase; Phosphatidylinositol 4; Phosphatidylinositol polyphosphate 5 phosphatase type IV; Phosphatidylinositol polyphosphate 5-phosphatase type IV; PPI5PIV;
抗原和靶标
- Q9NRR6 INP5E_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSKAENLRPSEPAPQPPEGRTLQGQLPGAPPAQRAGSPPDAPGSESPALACSTPATPSGEDPPARAAPIAPRPPARPRLERALSLDDKGWRRRRFRGSQEDLEARNGTSPSRGSVQSEGPGAPAHSCSPPCLSTSLQEIPKSRGVLSSERGSPSSGGNPLSGVASSSPNLPHRDAAVAGSSPRLPSLLPPRPPPALSLDIASDSLRTANKVDSDLADYKLRAQPLLVRAHSSLGPGRPRSPLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGADELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLYVIGVQEGCSDRREWETRLQETLGPHYVLLSSAAHGVLYMSLFIRRDLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLFITSHFTSGDGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEVFWFGDFNFRLSGGRTVVDALLCQGLVVDVPALLQHDQLIREMRKGSIFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYTDRVLYRSRHKGDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFDRELYLLGIKRRISKEIQRQQALQSQNSSTICSVS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NRR6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S38 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| Y293 | Phosphorylation | Uniprot | |
| Y439 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| T542 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot |
研究背景
Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (By similarity). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (By similarity).
Cytoplasm>Cytoskeleton>Cilium axoneme. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle. Cytoplasm.
Note: Peripheral membrane protein associated with Golgi stacks.
Detected in brain, heart, pancreas, testis and spleen.
Interacts (when prenylated) with PDE6D; this is important for normal location in cilia.
Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type IV family.
研究领域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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