AFF4 Antibody - #DF9180

>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(2)  
产品: AFF4 抗体
货号: DF9180
描述: Rabbit polyclonal antibody to AFF4
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Chicken
分子量: 127kDa; 127kD(Calculated).
蛋白号: Q9UHB7
RRID: AB_2842376

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
AFF4 Antibody detects endogenous levels of total AFF4.
RRID:
AB_2842376
引用格式: Affinity Biosciences Cat# DF9180, RRID:AB_2842376.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AF4/FMR2 family member 4; AF5Q31; Alf4; ALL1 fused gene from chromosome 5q31; HSPC092; Laf4; Lymphoid nuclear protein related to AF4 like; Major CDK9 elongation factor associated protein; MCEF; MGC107469; RP23-226J16.7;

抗原和靶标

免疫原:
Uniprot:

Q9UHB7(AFF4_HUMAN) >>Visit HPA database.

基因/基因ID:
AFF4(27125)
表达:
Q9UHB7 AFF4_HUMAN:

Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.

序列:
MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UHB7 作为底物

Site PTM Type Enzyme
S31 Phosphorylation
S32 Phosphorylation
Y39 Phosphorylation
S49 Phosphorylation
K79 Acetylation
S90 Phosphorylation
K107 Ubiquitination
T109 Phosphorylation
T117 Phosphorylation
S120 Phosphorylation
S124 Phosphorylation
S132 Phosphorylation
S133 Phosphorylation
T136 Phosphorylation
S155 Phosphorylation
Y156 Phosphorylation
S161 Phosphorylation
S163 Phosphorylation
K166 Acetylation
S176 Phosphorylation
S178 Phosphorylation
S179 Phosphorylation
S180 Phosphorylation
S188 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation
S210 Phosphorylation
S212 Phosphorylation
S222 Phosphorylation
S224 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S265 Phosphorylation
K288 Ubiquitination
K292 Ubiquitination
K297 Ubiquitination
K299 Ubiquitination
S302 Phosphorylation
S308 Phosphorylation
S310 Phosphorylation
S314 Phosphorylation
K321 Ubiquitination
T332 Phosphorylation
T336 Phosphorylation
K344 Ubiquitination
K350 Ubiquitination
T359 Phosphorylation
K363 Ubiquitination
S371 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
S389 Phosphorylation
S392 Phosphorylation
S440 Phosphorylation
S487 Phosphorylation
S490 Phosphorylation
S491 Phosphorylation
S494 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation
Y502 Phosphorylation
K504 Acetylation
T511 Phosphorylation
S514 Phosphorylation
T516 Phosphorylation
T518 Phosphorylation
S519 Phosphorylation
S526 Phosphorylation
T528 Phosphorylation
S533 Phosphorylation
S549 Phosphorylation
S553 Phosphorylation
K583 Sumoylation
T588 Phosphorylation
S599 Phosphorylation
S619 Phosphorylation
S620 Phosphorylation
Y668 Phosphorylation
S671 Phosphorylation
T674 Phosphorylation
K677 Ubiquitination
S679 Phosphorylation
S680 Phosphorylation
S686 Phosphorylation
S694 Phosphorylation
S703 Phosphorylation
S706 Phosphorylation
Y712 Phosphorylation
K747 Acetylation
S789 Phosphorylation
S800 Phosphorylation
S801 Phosphorylation
S804 Phosphorylation
S814 Phosphorylation
S821 Phosphorylation
K822 Acetylation
T834 Phosphorylation
S836 Phosphorylation
S839 Phosphorylation
S840 Phosphorylation
S845 Phosphorylation
S847 Phosphorylation
K849 Acetylation
T867 Phosphorylation
T871 Phosphorylation
S872 Phosphorylation
S873 Phosphorylation
S874 Phosphorylation
S875 Phosphorylation
K905 Ubiquitination
R911 Methylation
R935 Methylation
S963 Phosphorylation
S965 Phosphorylation
T973 Phosphorylation
Y986 Phosphorylation
T999 Phosphorylation
K1026 Ubiquitination
S1039 Phosphorylation
S1043 Phosphorylation
S1048 Phosphorylation
S1055 Phosphorylation
S1058 Phosphorylation
S1062 Phosphorylation
S1066 Phosphorylation
Y1069 Phosphorylation
K1121 Ubiquitination

研究背景

功能:

Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

细胞定位:

Nucleus.
Note: Associates to transcriptionally active chromatin but not at snRNA genes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.

亚基结构:

Interacts with ELL3; the interaction is direct (By similarity). Interacts with ELL2; the interaction is direct and leads to stabilize ELL2 and prevent ELL2 ubiquitination and degradation. Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL.

蛋白家族:

Belongs to the AF4 family.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.