产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9182, RRID:AB_2842378.
展开/折叠
AAA; AAAS; AAASb; Achalasia adrenocortical insufficiency alacrimia (Allgrove triple A); Achalasia adrenocortical insufficiency alacrimia; ADRACALA; ADRACALIN; Aladin; Allgrove triple A; DKFZp586G1624; GL003;
抗原和靶标
Widely expressed (PubMed:11159947, PubMed:16022285). Particularly abundant in cerebellum, corpus callosum, adrenal gland, pituitary gland, gastrointestinal structures and fetal lung (PubMed:11159947).
- Q9NRG9 AAAS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCSLGLFPPPPPRGQVTLYEHNNELVTGSSYESPPPDFRGQWINLPVLQLTKDPLKTPGRLDHGTRTAFIHHREQVWKRCINIWRDVGLFGVLNEIANSEEEVFEWVKTASGWALALCRWASSLHGSLFPHLSLRSEDLIAEFAQVTNWSSCCLRVFAWHPHTNKFAVALLDDSVRVYNASSTIVPSLKHRLQRNVASLAWKPLSASVLAVACQSCILIWTLDPTSLSTRPSSGCAQVLSHPGHTPVTSLAWAPSGGRLLSASPVDAAIRVWDVSTETCVPLPWFRGGGVTNLLWSPDGSKILATTPSAVFRVWEAQMWTCERWPTLSGRCQTGCWSPDGSRLLFTVLGEPLIYSLSFPERCGEGKGCVGGAKSATIVADLSETTIQTPDGEERLGGEAHSMVWDPSGERLAVLMKGKPRVQDGKPVILLFRTRNSPVFELLPCGIIQGEPGAQPQLITFHPSFNKGALLSVGWSTGRIAHIPLYFVNAQFPRFSPVLGRAQEPPAGGGGSIHDLPLFTETSPTSAPWDPLPGPPPVLPHSPHSHL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NRG9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C2 | Acetylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| T519 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot |
研究背景
Plays a role in the normal development of the peripheral and central nervous system. Required for the correct localization of aurora kinase AURKA and the microtubule minus end-binding protein NUMA1 as well as a subset of AURKA targets which ensures proper spindle formation and timely chromosome alignment.
Nucleus>Nuclear pore complex. Cytoplasm>Cytoskeleton>Spindle pole.
Note: In metaphase cells localizes within the spindle with some accumulation around spindle poles, with the highest concentration between the centrosome and metaphase plate (PubMed:26246606). The localization to the spindle is microtubule-mediated (PubMed:26246606).
Widely expressed. Particularly abundant in cerebellum, corpus callosum, adrenal gland, pituitary gland, gastrointestinal structures and fetal lung.
Interacts with NDC1, the interaction is required for nuclear pore localization. Interacts with the inactive form aurora kinase AURKA.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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