MT-ATP6 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from Rat muscle, using MT-ATP6 Antibody.

$Figure 4 CHCHD2 promotes F1F0-ATPase assembly in SH-SY5Y cells treated with MPP+.Flag-vector: LV-Flag-vector (Ubi-MCS-3FLAG-SV40-puromycin vector)-transfected SH-SY5Y cells; CHCHD2-Flag: LV-CHCHD2-Flag–transfected SH-SY5Y cells; CHCHD2-T61I: LV-CHCHD2-T61I-Flag-transfected SH-SY5Y cells; untreated: cells cultured in MEM-F12 medium; MPP+-treated: cells cultured with MPP+ (500 μM for 24 hours). (A) Representative western blots of ATP5A1, ATP6, VDAC1, and α-tubulin in the cytoplasm and mitochondrial membrane fractions without MPP+ treatment. (B) The ratio of ATP5A1 in the mitochondrial membrane and cytoplasmic fractions of control cells. (C) The ratio of ATP6 in the mitochondrial membrane and cytoplasmic fractions of control cells. (D) Representative western blots of ATP5A1, ATP6, and β-actin in whole-cell lysates from cells without MPP+ treatment. (E) Quantification of ATP5A1 and ATP6 expression in whole-cell lysates of control cells. (F) Representative western blots of ATP5A1, ATP6, VDAC1, and α-tubulin in the cytoplasmic and mitochondrial membrane fractions of cells treated with MPP+. (G) The ratio of ATP5A1 in the mitochondrial membrane and cytoplasmic fractions of MPP+-treated cells. (H) The ratio of ATP6 in the mitochondrial membrane and cytoplasmic fractions of MPP+-treated cells. (I) Representative western blots of ATP5A1, ATP6, and β-actin in whole-cell lysates of cells treated with MPP+. (J) The relative expression levels of ATP5A1 and ATP6 in whole-cell lysates of MPP+-treated cells. Data are expressed as the mean ± SD (n = 3 independent experiments). **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). Mito-mem: Mitochondrial membrane; MPP+: 1-methyl-4-phenylpyridinium; VDAC1: voltage-dependent anion-selective channel protein 1.$

Figure 4 CHCHD2 promotes F1F0-ATPase assembly in SH-SY5Y cells treated with MPP+.

产品:	MT-ATP6 抗体
货号:	DF9237
描述:	Rabbit polyclonal antibody to MT-ATP6
应用:	WB IF/ICC
反应:	Human, Rat
分子量:	25 kDa; 25kD(Calculated).
蛋白号:	P00846
RRID:	AB_2842433

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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MS Report

HPLC Report

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Rat

克隆:

Polyclonal

特异性:

MT-ATP6 Antibody detects endogenous levels of total MT-ATP6.

RRID:

AB_2842433
引用格式: Affinity Biosciences Cat# DF9237, RRID:AB_2842433.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

ATP synthase subunit a; ATP6; ATP6_HUMAN; ATPASE6; F-ATPase protein 6; MT-ATP6; MTATP6;

抗原和靶标

免疫原:

Uniprot:

P00846(ATP6_HUMAN) >>Visit HPA database.

基因/基因ID:

MT-ATP6(4508)

序列:

P00846 ATP6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNENLFASFIAPTILGLPAAVLIILFPPLLIPTSKYLINNRLITTQQWLIKLTSKQMMTMHNTKGRTWSLMLVSLIIFIATTNLLGLLPHSFTPTTQLSMNLAMAIPLWAGTVIMGFRSKIKNALAHFLPQGTPTPLIPMLVIIETISLLIQPMALAVRLTANITAGHLLMHLIGSATLAMSTINLPSTLIIFTILILLTILEIAVALIQAYVFTLLVSLYLHDNT

研究背景

P00846_ATP6_HUMAN

功能:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.

细胞定位:

Mitochondrion inner membrane>Multi-pass membrane protein.

亚基结构:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity). Interacts with DNAJC30; interaction is direct.

蛋白家族:

Belongs to the ATPase A chain family.

研究领域

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson’s disease. Neural Regeneration Research, 2024 (PubMed: 37488867) [IF=6.1]

Application: WB Species: Human Sample: SH-SY5Y cells

Figure 4 CHCHD2 promotes F1F0-ATPase assembly in SH-SY5Y cells treated with MPP+.Flag-vector: LV-Flag-vector (Ubi-MCS-3FLAG-SV40-puromycin vector)-transfected SH-SY5Y cells; CHCHD2-Flag: LV-CHCHD2-Flag–transfected SH-SY5Y cells; CHCHD2-T61I: LV-CHCHD2-T61I-Flag-transfected SH-SY5Y cells; untreated: cells cultured in MEM-F12 medium; MPP+-treated: cells cultured with MPP+ (500 μM for 24 hours). (A) Representative western blots of ATP5A1, ATP6, VDAC1, and α-tubulin in the cytoplasm and mitochondrial membrane fractions without MPP+ treatment. (B) The ratio of ATP5A1 in the mitochondrial membrane and cytoplasmic fractions of control cells. (C) The ratio of ATP6 in the mitochondrial membrane and cytoplasmic fractions of control cells. (D) Representative western blots of ATP5A1, ATP6, and β-actin in whole-cell lysates from cells without MPP+ treatment. (E) Quantification of ATP5A1 and ATP6 expression in whole-cell lysates of control cells. (F) Representative western blots of ATP5A1, ATP6, VDAC1, and α-tubulin in the cytoplasmic and mitochondrial membrane fractions of cells treated with MPP+. (G) The ratio of ATP5A1 in the mitochondrial membrane and cytoplasmic fractions of MPP+-treated cells. (H) The ratio of ATP6 in the mitochondrial membrane and cytoplasmic fractions of MPP+-treated cells. (I) Representative western blots of ATP5A1, ATP6, and β-actin in whole-cell lysates of cells treated with MPP+. (J) The relative expression levels of ATP5A1 and ATP6 in whole-cell lysates of MPP+-treated cells. Data are expressed as the mean ± SD (n = 3 independent experiments). **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). Mito-mem: Mitochondrial membrane; MPP+: 1-methyl-4-phenylpyridinium; VDAC1: voltage-dependent anion-selective channel protein 1.

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MT-ATP6 Antibody - #DF9237