| 产品: | ATP5O 抗体 |
| 货号: | DF9242 |
| 描述: | Rabbit polyclonal antibody to ATP5O |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 23 kDa; 23kD(Calculated). |
| 蛋白号: | P48047 |
| RRID: | AB_2842438 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9242, RRID:AB_2842438.
展开/折叠
ATP synthase O subunit mitochondrial precursor; ATP synthase subunit O; ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; ATP5O; ATPO; ATPO_HUMAN; mitochondrial; Mitochondrial ATP synthase, O subunit; Oligomycin sensitivity conferral protein; OSCP;
抗原和靶标
- P48047 ATPO_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPAVSGLSRQVRCFSTSVVRPFAKLVRPPVQVYGIEGRYATALYSAASKQNKLEQVEKELLRVAQILKEPKVAASVLNPYVKRSIKVKSLNDITAKERFSPLTTNLINLLAENGRLSNTQGVVSAFSTMMSVHRGEVPCTVTSASPLEEATLSELKTVLKSFLSQGQVLKLEAKTDPSILGGMIVRIGEKYVDMSVKTKIQKLGRAMREIV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P48047 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R29 | Methylation | Uniprot | |
| Y35 | Phosphorylation | Uniprot | |
| Y41 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K60 | Acetylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| R64 | Methylation | Uniprot | |
| K70 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| K98 | Acetylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K162 | Acetylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y193 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot |
研究背景
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Acetylation at Lys-162 decreases ATP production. Deacetylated by SIRT3.
Mitochondrion. Mitochondrion inner membrane.
F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).
Belongs to the ATPase delta chain family.
研究领域
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
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