产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9259, RRID:AB_2842455.
展开/折叠
ATP-dependent RNA helicase DDX42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box polypeptide 42 protein; DEAD box protein 42; EC 3.6.1.-; FLJ43179; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD-box protein; SF3b125; SF3b125 DEAD-box protein; Splicing factor 3B-associated 125 kDa protein;
抗原和靶标
Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.
- Q86XP3 DDX42_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86XP3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Acetylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K50 | Methylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K145 | Acetylation | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K270 | Sumoylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| K605 | Ubiquitination | Uniprot | |
| K626 | Acetylation | Uniprot | |
| R673 | Methylation | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| K686 | Acetylation | Uniprot | |
| K700 | Methylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| Y707 | Phosphorylation | Uniprot | |
| K708 | Methylation | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| T742 | Phosphorylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| K806 | Acetylation | Uniprot | |
| R814 | Methylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| Y844 | Phosphorylation | Uniprot | |
| R875 | Methylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| K899 | Sumoylation | Uniprot | |
| S914 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot |
研究背景
ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
Cytoplasm. Nucleus speckle. Nucleus>Cajal body.
Note: Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.
Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.
Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. Interacts (via the C-terminus) with TP53BP2; the interaction is not inhibitied by TP53BP2 ubiquitination and is independent of p53/TP53.
Belongs to the DEAD box helicase family. DDX42 subfamily.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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