B4GALT4 Antibody - #DF9274

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产品: B4GALT4 抗体
货号: DF9274
描述: Rabbit polyclonal antibody to B4GALT4
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit
蛋白号: O60513
RRID: AB_2842470

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
B4GALT4 Antibody detects endogenous levels of total B4GALT4.
RRID:
AB_2842470
引用格式: Affinity Biosciences Cat# DF9274, RRID:AB_2842470.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

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B4GALT4; B4Gal T4; B4Gal-T4; Beta 1 4 galactosyltransferase 4; Beta 1 4 GalTase 4; Beta N acetylglucosaminyl glycolipid beta 1 4 galactosyltransferase 4; beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4; Beta4Gal T4; beta4Gal-T4; EC 2.4.1.-; UDP Gal:betaGlcNAc beta ,4 galactosyltransferase 4; UDP Gal:betaGlcNAc beta 1 4 galactosyltransferase polypeptide 4; UDP galactose:beta N acetylglucosamine beta 1 4 galactosyltransferase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4;

抗原和靶标

免疫原:

A synthesized peptide derived from human B4GALT4, corresponding to a region within the internal amino acids.

基因/基因ID:
B4GALT4(8702)

研究领域

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. JOURNAL OF CELLULAR PHYSIOLOGY, 2022 (PubMed: 34270095) [IF=4.5]

Application: WB    Species: Human    Sample: HCC cell

FIGURE 8 Effect of B4GALT4 knockdown on cell migration and invasion in HCC. SMMC‐7721 (a) and HepG2 (b) cells were transiently transfected with scramble siRNA (negative control) and B4GALT4 siRNA for 48h. B4GALT4 expression in two HCC cell lines were detected by western blot analysis in a nontreated control, scramble control, and B4GALT4 knockdown cells. Wound‐healing assays validating the effects of B4GALT4 knockdown on SMMC‐7721 (c) and HepG2 (d) cells migration. Scale bars, 100mm. Transwell assays validated the effects of B4GALT4 knockdown on SMMC‐7721 (e) and HepG2 (f) cells invasion. Scale bars, 200mm. (g, h) The statistical analysis of cell relative migration level. (i, j) The statistical analysis of cell relative invasiveness level. (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001). B4GALT, beta 1,4‐galactosyltransferase; HCC, hepatocellular carcinoma; siRNA, small interfering RNA

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