B4GALT4 Antibody - #DF9274

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产品: B4GALT4 抗体
货号: DF9274
描述: Rabbit polyclonal antibody to B4GALT4
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 40 kDa; 40kD(Calculated).
蛋白号: O60513
RRID: AB_2842470

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%)
克隆:
Polyclonal
特异性:
B4GALT4 Antibody detects endogenous levels of total B4GALT4.
RRID:
AB_2842470
引用格式: Affinity Biosciences Cat# DF9274, RRID:AB_2842470.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B4GALT4; B4Gal T4; B4Gal-T4; Beta 1 4 galactosyltransferase 4; Beta 1 4 GalTase 4; Beta N acetylglucosaminyl glycolipid beta 1 4 galactosyltransferase 4; beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4; Beta4Gal T4; beta4Gal-T4; EC 2.4.1.-; UDP Gal:betaGlcNAc beta ,4 galactosyltransferase 4; UDP Gal:betaGlcNAc beta 1 4 galactosyltransferase polypeptide 4; UDP galactose:beta N acetylglucosamine beta 1 4 galactosyltransferase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4;

抗原和靶标

免疫原:
Uniprot:

O60513(B4GT4_HUMAN) >>Visit HPA database.

基因/基因ID:
B4GALT4(8702)
表达:
O60513 B4GT4_HUMAN:

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

序列:
MGFNLTFHLSYKFRLLLLLTLCLTVVGWATSNYFVGAIQEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPQECKALQRVAILVPHRNREKHLMYLLEHLHPFLQRQQLDYGIYVIHQAEGKKFNRAKLLNVGYLEALKEENWDCFIFHDVDLVPENDFNLYKCEEHPKHLVVGRNSTGYRLRYSGYFGGVTALSREQFFKVNGFSNNYWGWGGEDDDLRLRVELQRMKISRPLPEVGKYTMVFHTRDKGNEVNAERMKLLHQVSRVWRTDGLSSCSYKLVSVEHNPLYINITVDFWFGA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
82
Horse
82
Bovine
82
Sheep
82
Rabbit
82
Dog
70
Xenopus
67
Chicken
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60513 作为底物

Site PTM Type Enzyme
S88 Phosphorylation
Y155 Phosphorylation
Y158 Phosphorylation
Y178 Phosphorylation

研究背景

功能:

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

细胞定位:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein.
Note: Trans cisternae of Golgi stack.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

蛋白家族:

Belongs to the glycosyltransferase 7 family.

研究领域

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. JOURNAL OF CELLULAR PHYSIOLOGY, 2022 (PubMed: 34270095) [IF=5.6]

Application: WB    Species: Human    Sample: HCC cell

FIGURE 8 Effect of B4GALT4 knockdown on cell migration and invasion in HCC. SMMC‐7721 (a) and HepG2 (b) cells were transiently transfected with scramble siRNA (negative control) and B4GALT4 siRNA for 48h. B4GALT4 expression in two HCC cell lines were detected by western blot analysis in a nontreated control, scramble control, and B4GALT4 knockdown cells. Wound‐healing assays validating the effects of B4GALT4 knockdown on SMMC‐7721 (c) and HepG2 (d) cells migration. Scale bars, 100mm. Transwell assays validated the effects of B4GALT4 knockdown on SMMC‐7721 (e) and HepG2 (f) cells invasion. Scale bars, 200mm. (g, h) The statistical analysis of cell relative migration level. (i, j) The statistical analysis of cell relative invasiveness level. (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001). B4GALT, beta 1,4‐galactosyltransferase; HCC, hepatocellular carcinoma; siRNA, small interfering RNA

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