产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9324, RRID:AB_2842520.
展开/折叠
CALR 3; CALR3; CALR3_HUMAN; Calreticulin 2; Calreticulin-2; Calreticulin-3; Calreticulin2; Calreticulin3; Calsperin; Cancer/testis antigen 93; CMH19; CRT2; CT93; FLJ25355; MGC26577;
抗原和靶标
- Q96L12 CALR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARALVQLWAICMLRVALATVYFQEEFLDGEHWRNRWLQSTNDSRFGHFRLSSGKFYGHKEKDKGLQTTQNGRFYAISARFKPFSNKGKTLVIQYTVKHEQKMDCGGGYIKVFPADIDQKNLNGKSQYYIMFGPDICGFDIKKVHVILHFKNKYHENKKLIRCKVDGFTHLYTLILRPDLSYDVKIDGQSIESGSIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKDVWLHRKMKNTDYLTQYDLSEFENIGAIGLELWQVRSGTIFDNFLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLSGKINRHEHYFNQFHRRNEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96L12 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y57 | Phosphorylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| K230 | Acetylation | Uniprot | |
| Y374 | Phosphorylation | Uniprot |
研究背景
During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity). CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.
Endoplasmic reticulum lumen.
Testis specific.
Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Belongs to the calreticulin family.
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