Phospho-Insulin Receptor beta (Tyr1361) Antibody - #AF3099

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产品: 磷酸化 Insulin Receptor beta (Tyr1361) 抗体
货号: AF3099
描述: Rabbit polyclonal antibody to Phospho-Insulin Receptor beta (Tyr1361)
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P06213
RRID: AB_2834536

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-IR (Tyr1361) Antibody detects endogenous levels of IR only when phosphorylated at Tyrosine 1361.
RRID:
AB_2834536
引用格式: Affinity Biosciences Cat# AF3099, RRID:AB_2834536.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD220; HHF5; human insulin receptor; Insr; INSR_HUMAN; Insulin receptor subunit beta; IR 1; IR; IR-1; IR1;

抗原和靶标

免疫原:

A synthesized peptide derived from human IR around the phosphorylation site of Tyr1361.

基因/基因ID:
INSR(3643)
描述:
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling. Cell death & disease, 2023 (PubMed: 37365185) [IF=8.1]
2). Curcumin suppresses JNK pathway to attenuate BPA-induced insulin resistance in LO2 cells. BIOMEDICINE & PHARMACOTHERAPY, 2018 (PubMed: 29793316) [IF=6.9]
3). Low molecular weight fucoidan restores diabetic endothelial glycocalyx by targeting neuraminidase2: A new therapy target in glycocalyx shedding. British journal of pharmacology, 2023 (PubMed: 37994102) [IF=6.8]
4). 海马胰岛素抵抗与糖尿病诱发认知功能障碍中的Sirtuin 1信号通路. 中国神经再生研究(英文版), 2021 [IF=5.9]
5). Hippocampal insulin resistance and the Sirtuin 1 signaling pathway in diabetes-induced cognitive dysfunction. Neural Regeneration Research, 2021 (PubMed: 33907035) [IF=5.9]

Application: WB    Species: Rat    Sample: diabetic model rats

Figure 5 Insulin signaling molecules and SIRT1 expression are reduced in diabetic rats at week 8 of diabetes. (A) Bands of p-IR, p-IRS-1, and SIRT1. (B–D) Relative expression levels of p-IR (B), p-IRS-1 (C), and SIRT1 (D). The relative expression levels are expressed as the optical density ratio compared against GAPDH levels. Data are presented as the mean ± SEM (n = 6 per group). **P < 0.01 (one-way analysis of variance). The experiments were repeated three times. DIA-8W: Diabetic rats at week 8 of diabetes; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IR: insulin receptor; IRS-1: IR receptor substrate 1; NS: non-streptozotocin rats; p-IR: phospho-insulin receptor; p-IRS-1: phospho-insulin receptor substrate; SIRT1: Sirtuin 1.
6). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences, 2023 (PubMed: 38146533) [IF=5.0]

Application: WB    Species: Human    Sample: adipose tissue

FIGURE 11 Involvement of SHBG in the insulin signaling pathway. Relative expression levels of PI3K (A), AKT1 (B), GLUT4 (C), IRS1 (D), IRS2 (E), INSR (F) were analyzed by qRT-PCR. Protein levels of PI3K (G), AKT (H), GLUT4 (I), IRS1 (J), IRS2 (K), SHBG (L), IR (M) were analyzed by Western Blot. Representative immunoblots (N). Representative data are shown as mean ± SD.
7). Silencing of ANGPTL8 Alleviates Insulin Resistance in Trophoblast Cells. Frontiers in Endocrinology, 2021 (PubMed: 34163433) [IF=3.9]

Application: WB    Species: mouse    Sample: placenta

FIGURE 1 | Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice.(J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRb(Tyr1361), IRb, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues.

Application: WB    Species: Mice    Sample: serum and placenta tissues

Figure 1 Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice. (A) The mice were treated as described in the chart. (B) The body weight of mice in normal fat diet (NFD) and high fat diet (HFD) groups. (C) Oral glucose tolerance test (OGTT) was performed at gestational day (GD)0.5, 11.5 and 16.5. (D, E) Fasting blood glucose and insulin levels were measured at GD18.5. (F) Homeostasis model assessment insulin resistance (HOMA-IR) was calculated as follow: HOMA-IR= blood glucose (mM)×blood insulin (mU/l)/22.5. (G) The contents of triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) in serum were detected. (H) HE staining was performed to detect the pathological changes in labyrinth zone of placenta tissues. (I) Periodic acid Schiff (PAS) staining was carried out to detect the glycogen accumulation in labyrinth zone of placenta tissues. (J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRβ(Tyr1361), IRβ, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues. (K) The expression levels of glucose transporter 1 (GLUT1) and GLUT4 in placenta tissues. (L) The serum level of ANGPTL8 in mice. (M, N) The mRNA and protein levels of ANGPTL8 in placenta tissues. (the scale bar represents 100 μm; **p < 0.01, ***p < 0.001 vs. NFD).
8). Orphan nuclear receptor NUR77 relieves insulin resistance in HTR-8/SVneo trophoblast cells through activation of autophagy and insulin signaling. JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY, 2022 (PubMed: 35902547) [IF=3.7]
9). Curcumin attenuates BPA-induced insulin resistance in HepG2 cells through suppression of JNK/p38 pathways. TOXICOLOGY LETTERS, 2017 (PubMed: 28300666) [IF=2.9]

Application: WB    Species: human    Sample: HepG2

Application: WB    Species: human    Sample: HepG2 cells

Figure 5| Effect of curcumin on BPA-induced insulin resistance in HepG2 cells. (C) HepG2 cells were treated with combination of 100 nM BPA and different concentrations of curcumin (1 µM to 5 µM) for 5 d, the levels of p-IR, p-IRS1 and p-AKT were measured by Western blot. Data are expressed as mean ± SD. ∗∗P < 0.01, significantly different as compared with the untreated control, # P< 0.05, significantly different as compared with BPA group.
10). The role of Smad4 in the regulation of insulin resistance, inflammation and cell proliferation in HTR8‐Svneo cells. CELL BIOCHEMISTRY AND FUNCTION, 2021 (PubMed: 33079408) [IF=2.8]

Application: WB    Species: Human    Sample: human insulin resistance

FIGURE 2 The deficiency of Smad4 elevated the insulin sensitivity in a cellular model of human insulin resistance. A, The validation of downregulated Smad4 by western-blot. B, The glucose consumption was determined by commercial kit. C-G, The expression levels of p-IRβTyr1361, IRβ, p-IRS-1Ser307, p-IRS-1Tyr612, IRS-1,Akt and p-AktSer473 were determined by western-blot along with relative quantification. H and I, The expressions of GLUT1 and GLUT4 were determined by western-blot along with relative quantification. Values are expressed as mean ± SD. n = 3. *P < .05, **P < .01, ***P < .001, ****P < .0001

Application: WB    Species: human    Sample: HTR-8/SVneocells

FIGURE 2 |The deficiency of Smad4 elevated the insulin sensitivity in a cellular model of human insulin resistance.C-G, The expression levels of p-IRβTyr1361, IRβ,p-IRS-1Ser307, p-IRS-1Tyr612, IRS-1,Akt and p-AktSer473 were determined by western-blot along with relative quantification.
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